Acquisition of a Differential Scanning Calorimeter

我们需要一种差示扫描量热计（DSC），配备扩展温度范围（220摄氏度），外部冷却装置，数据收集和分析系统以及软件和可重复使用的电池。这是提供的唯一具有必要灵敏度和温度范围的DSC。该仪器的收购将允许私家侦探s分析了嗜热生物和超嗜热生物中酶的热变性特性，研究了已知结构的小蛋白热变性的分子机制；分析一种具有生理意义的蛋白酶抑制剂作用的热力学基础，探讨亚基和结构域相互作用在酶活性产生中的意义。这些研究将扩展我们对蛋白质热稳定性和酶活性的结构基础的认识。

We are requesting for a differential scanning calorimeter ( DSC) equipped with extended temperature range (220C), an external cooling unit, data collection and analysis system and software and reusable cells. This is the only DSC with the requisite sensitivity and temperature range offered. The acquisition of this instrument will permit the P.I.'s to analyze the thermal denaturation characteristics of enzymes from thermophilic and superthermophillic organisms, to study the molecular mechanism of thermal denaturation of small proteins of know structure, to analyze thermodynamic basis of action of a protease inhibitor of physiological significance and to explore the significance of subunit and domain interactions in producing activity in an enzyme. These studies will extend our knowledge of the structural basis of thermal stability in proteins and activity in enzymes.