Acquisition of an Optima XL-A Analytical Ultracentrifuge

购买 Optima XL-A 分析超速离心机

• 批准号: 9317749
• 负责人: John Brewer
• 金额: $ 7.25万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-07-15 至 1996-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9317749&HistoricalAwards=false
• 关键词: Acquisition; Optima; XL; Analytical; Ultracentrifuge

We are requesting funds from the National Science Foundation to purchase an Analytical Ultracentrifuge. This is to replace our 26-year old Model E ultracentrifuge, which is nearly impossible to maintain and for which certain accessories are no longer produced. Also, the XL-A has greatly increased sensitivity as a result of a very much shorter optical path length and advances in component quality over the last quarter century. Furthermore, its output is now fully computer processed, enabling far more rapid and reliable processing of results. With this instrument, we will be able to obtain molecular weights, effective partial specific volumes and sedimentation constants. These pieces of information will allow us to determine molecular architecture of multisubunit assemblies, more reliably estimate compositions of macromolecular aggregates which are complex and which may interact extensively with solvent constituents, and obtain quantitative parameters of protein association reactions. Specifically, the instrument will be used to obtain "true" (in a thermodynamic sense) molecular weights of cellulase components, metalloenzymes and redox proteins from hyperthermophiles, explore subunit interactions in human choriogonadotropin, measure hydration and salt binding in yeast enolase and measure complex formation between reactants in the bacterial luciferase reaction.

我们正在向国家科学基金会申请资金，以购买分析超级计算机。 这是为了取代我们的26岁的E型ultracenthouge，这是几乎不可能的维护和某些配件不再生产。 此外，由于光程长度大大缩短以及过去四分之一个世纪来元件质量的进步，XL-A的灵敏度大大提高。 此外，其产出现在完全由计算机处理，从而能够更迅速和可靠地处理结果。 利用该仪器，我们将能够获得分子量、有效偏比容和沉降常数。 这些信息将使我们能够确定多亚基组装的分子结构，更可靠地估计复杂的大分子聚集体的组成，并可能与溶剂成分广泛相互作用，并获得蛋白质缔合反应的定量参数。 具体而言，该仪器将被用来获得“真正的”（在热力学意义上）的分子量的纤维素酶组分，金属酶和氧化还原蛋白从超嗜热菌，探索亚基相互作用在人绒毛膜促性腺激素，测量水合作用和盐结合在酵母烯醇化酶和测量细菌荧光素酶反应中的反应物之间的复合物形成。