The Use of Immunocytochemical Techniques for Phytoplankton Growth Rate Estimation Via Cell Cycle Analysis

使用免疫细胞化学技术通过细胞周期分析估计浮游植物生长速率

• 批准号: 9115618
• 负责人: Gordon Taylor
• 金额: $ 27.88万
• 依托单位: SUNY at Stony Brook
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-03-01 至 1996-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9115618&HistoricalAwards=false
• 关键词: Use; Immunocytochemical; Techniques; Phytoplankton; Growth

Single-cell techniques for measuring species-specific growth rates and quantum yield in situ have been developed and tested. The growth rates of individual species of marine phytoplankton can be determined from data on a species' diel cycle of DNA replication. The species-specific quantum yield is determined from single-cell absorption spectra and the spectral distribution of daily integrated light intensity. These techniques have been tested in field conditions, and the results agree with related lab studies. In this research, Dr. Carpenter and collaborators will develop a simpler and more widely applicable method of measuring growth rates by using cell cycle markers. This new approach will be less time consuming, eliminate the requirement for sophisticated equipment, and allow the production of permanent slides for record keeping. A technique for separating phytoplankton cells from zooplankton and detrital particles will be developed to make these measurements possible. In addition, these techniques will be applied to study the causes of phytoplankton succession in Long Island Sound and to test the hypothesis that the change from a diatom dominated community to a dinoflagellate dominated one is caused by the alteration of intrinsic growth rates in corresponding populations.

测量物种特异性生长的单细胞技术 速率和量子产率在原位已经开发和测试。 海洋浮游植物个体的生长率 可以从一个物种的DNA昼夜循环数据中确定 复制的 确定了物种特异性量子产率 从单细胞吸收光谱和光谱 日综合光照强度分布。 这些 技术已在现场条件下进行了测试，结果 同意相关实验室研究。 在这项研究中，卡彭特博士和合作者将 开发一种更简单和更广泛适用的测量方法 通过使用细胞周期标志物的生长速率。 这种新方法将 更少的时间消耗，消除对 先进的设备，并允许生产永久 用于记录保存的幻灯片。 一种分离技术， 浮游动物和碎屑颗粒中的浮游植物细胞 使这些测量成为可能。 此外，本发明还提供了一种方法， 这些技术将用于研究 长岛海峡的浮游植物演替，并测试 假设从硅藻占主导地位的社区到 甲藻占优势的一个是由改变 相应人群的内在增长率。