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Immunocytochemical Study of the Functional Development and Differentiation in the Photoreceptor Organs of Fishes

鱼类感光器官功能发育和分化的免疫细胞化学研究

基本信息

批准号：
12660167
负责人：
OMURA Yuri
金额：
$ 2.37万
依托单位：
Graduate School of Bioagricultural Science, Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

(1) In the retinas of the glass eel Anguilla japonica and the young goldfish Carassius auratus, extremely intense immunostaining of taurine was found in the cone outer segments, rod inner segments, photoreceptor supranuctear region and outer plexifom layer. The rod outer segments were not immunostained in the light-adapted state, while they were intensely immunostained in the dark-adapted state. Consequently, it was suggested that the lack of immunoreactivity in the rod outer segment may depend on light stimulation. In addition, the conspicuous immunocytochemical localization of taurine was discussed with the possible fonctional rotes for taurine in the fish retina.(2.) The proliferation of rod photoreceptor cells was investigated in the retina of Japanese eel, elvers from the coastal sea, adults from the river, and migrating adults from the ocean by means of histological and immunocylochemical methods. We found an increase in the ratio between the total nuclear number in the outer nuc … More lear layer and the number of cone cells, and in the number of rod outer segment layers (including inner segments), and examined PCNA (proliferating cell nuclear antigen)-immunoreactive cells in the outer and inner nuclear layers in adult eel from fee river. The results suggested that rod photoreceptor cells proliferate in the eel retina throughout the life.(3) Developing, mature and/or adult retinae of the lefteye flounder Paralichthys olivaceus were examined by means of light- and electron microscopic and immunocytochemical methods. A great number of putative precursors and/or progenitors were found in or progenitors the outer nuclear layer of the metamorphosing and juvenile retinae. In addition, a tremendous number of PCNA-immunoreactive cells were demonstrated in the outer nuclear layer in mature and/or adult retinae. These findings suggested that rod photoreceptor cells proliferate in the mature retina of lefteye flounder, perhaps due to the dramatic change in their visual habitats from the surface to the bottom waters. Less

(1)在玻璃鳗鲡（Anguillajaponica）和幼金鱼（Carassiusauratus）的视网膜中，牛磺酸在视锥细胞外节、视杆细胞内节、感光细胞核上区和外丛状层中有极强的免疫染色。在光适应状态下，视杆外节没有免疫染色，而在暗适应状态下，它们被强烈的免疫染色。因此，有人建议，在杆外段缺乏免疫反应性可能取决于光刺激。此外，还讨论了牛磺酸在鱼视网膜中的免疫细胞化学定位及其可能的作用机制。（2.）本文用组织学和免疫细胞化学方法研究了日本鳗鲡、沿海幼鳗、河鳗成鱼和海洋洄游成鱼视网膜视杆细胞的增殖。我们发现外核的总核数与 ...更多信息结果表明：（1）幼鳗的视锥细胞数、视杆细胞数、视杆细胞外节层数（包括内节）、视杆细胞外节层数、视杆细胞内节层数、视杆细胞外节层数、视杆细胞外节层数、视杆结果表明，视杆细胞在鳗鲡视网膜中的增殖是终生的。(3)用光镜、电镜和免疫细胞化学方法对褐牙鲆（Paralichthys olivaceus）视网膜的发育、成熟和/或成体进行了观察。在变态期和幼年期视网膜的外核层中发现了大量的前体细胞和/或前体细胞。此外，大量的PCNA免疫反应细胞被证明在成熟和/或成年视网膜的外核层。这些结果表明，视杆细胞增殖在成熟的左眼比目鱼的视网膜，可能是由于其视觉栖息地的急剧变化，从表面到底部的沃茨。少