Regulation of gene expression by oxygen in Escherichia coli

该提案的目的是研究大肠杆菌中hbp基因的调节。hbp基因产物在氨基酸序列上与来自玻璃体菌属物种的血红蛋白样蛋白非常相似，并且与玻璃体菌属蛋白一样，其响应于环境氧浓度而被调节。该提案的具体目标是：1。野生型和突变株hbp基因调控的生理学特征。2. hbp基因表达的遗传学特征。3.从hbp启动子鉴定和表征hbp调节所需的序列。从这项研究中获得的信息将有助于目前对基因表达响应环境因素的知识，也将有助于对基因调控的全面了解。

The objective of the proposal is to study the regulation of the hbp gene in Escherichia coli. The hbp gene product is very similar in amino sequence to a hemoglobin-like protein from a Vitreoscilla sp. and like the Vitreoscilla protein is regulated in response to environmental oxygen concentration. The specific aims of the proposal are: 1. Physiological characterization of hbp gene regulation in wild-type and mutant strains. 2. Genetic characterization of hbp gene expression. 3. Identify and characterize the sequence required for hbp regulation from the hbp promoter. The information obtained from this research will contribute to the present knowledge on gene expression in response to environmental factors, and will also contribute to the overall understanding of gene regulation.