Use of Microbial Assays to Distinguish Between Heavy Metal and Organic Chemical Toxicity

这是一个为研究提供更新支持的奖项 这是在NSF资助号88-13714下发起的， 其目的是确定 在选择的代谢过程的抑制， 用于生产酶的微生物可用于 预测有毒物质对微生物的影响 深层培养用生物，生物废水 治疗过程。 结果包括应用 短期生物测定开发的结果 重金属毒性测定方法 环境媒体。 该项目的目标包括努力增加 生物传感程序的灵敏度，通过改变 测试生物体细胞壁的渗透性。 工作将 包括努力简化程序的实施， 这将提高其在日常使用中潜力， 改善生物废水处理过程的操作， 研究其用于生物测定的潜力， 区分重金属和有机毒物， 在评价重金属毒性时， 与固体物质相关的污染物， 焚化产生的沉积物、土壤、污泥和灰烬 流程. 这项研究的结果预计将用于 生物处理工艺工程设计 受到有毒物质的影响， 评估废物排放对 在确定有毒物质对环境的影响时， 潜在的农业和工业用水再利用 目的，以及评估生物、化学、 和物理-补救所采用的程序 危险废弃物处理场。

This is a an award to provide renewal support for research that was initiated under NSF Grant No. 88-13714, objectives of which were to determine the conditions under which the inhibition of metabolic processes in selected microorganisms for production of enzymes could be used to predict the effect of toxic substances on microorganisms organisms used in submerged culture, biological wastewater treatment processes. Results included the application of findings to development of a short-term, bioassay procedure for determination of heavy metal toxicity in environmental media. Objectives of this project include efforts to increase the sensitivity of the biosensing procedure by altering the permeability of the test-organism's cell wall. Work will include efforts to simplify the conduct of the procedure that would enhance its potential for routine use in improving operation of biowastewater treatment processes, investigate its potential for use in bioassays to distinguish between heavy metal and organic toxicants, and in evaluation of heavy-metal toxicity associated with contaminants associated with solid substances such as sediments, soils, sludges and ash from incineration processes. Results of this research are expected to be utilized in the engineering design of biological treatment processes that are subjected to being impacted by toxicants, in assessing the impacts of waste discharges to the environment, in determining the impacts of toxicants on potential water reuse for agricultural and industrial purposes, and in evaluating results of bio-, chemical-, and physical- procedures that are applied to remediation of hazardous waste sites.