Assessment of the Toxicity and Endocrine Disruption Potential of Municipal, Construction and Demolition, and Industrial Solid Wastes

9906060 Bitton这一奖项旨在支持对垃圾填埋场产生的渗滤液的毒理学研究，这些垃圾填埋场含有来自垃圾填埋场的固体废物，这些垃圾填埋场含有市政和工业建筑和拆除碎片。本研究将对三个现有堆填区的渗滤液进行研究，以确定其急性毒性。其中制定了使用微生物测定法区分重金属和有机化学毒性的程序。在这个新项目中，将研究佛罗里达州Alachua、Citrus和Levy县现有固体废物填埋场的渗滤液的基线毒性，以确定其基线毒性，这些毒性在多大程度上归因于其重金属含量，并确定其内分泌干扰的潜力。渗滤液也将在模拟固体废物暴露于降雨的实验室柱中产生，并在使用缓冲液、去离子水和溶剂作为洗脱剂的批量程序中产生。这些渗滤液将通过土壤柱，以确定通过土壤达到的毒性衰减潜力。重组酵母将被用于检测混合固体废物中的环境雌激素，而黑头鲦鱼产生的卵黄蛋白将被用作评估内分泌干扰的生物标志物。来自建筑和拆除活动的固体废物通常被放置在工地和堆填区，这些地方没有衬砌，也没有设计或建造以防止或限制渗滤液污染土壤和地下水。这项研究的结果可能会提供知识，可用于确定填埋场的适当位置和工程设计，以避免垃圾填埋场渗滤液污染土壤，地表和地下水。预计这项工作还将验证可用于快速检测固体废物对环境资源的毒性、内分泌干扰和其他有害环境影响的潜在短期分析

9906060 Bitton This is an award to support research on toxicology of leachates emerging from landfills containing solid wastes originating from landfills that contain municipal and industrial construction and demolition debris. The leachates from three existing landfills will be studied to determine their acute toxicity applying procedures developed in research conducted under NSF Grant No. BES 91-17267 in which procedures were developed to use microbial assays to distinguish between heavy metal and organic chemical toxicity. In this new project, the baseline toxicity of leachates from existing solid waste landfills in Alachua, Citrus and Levy Counties in Florida will be studied to determine their baseline toxicities, the extent to which these are attributable to their content of heavy metals and to determine their potential for endocrine disruption. Leachates will also be produced in laboratory columns simulating exposure of solid wastes to rainfall and in batch procedures using buffers, deionized water and solvents as eluants. These leachates will be passed through soil columns to determine the toxicity attenuation potential achieved by passage through soil. A recombinant yeast will be used to detect environmental estrogens in mixed solid waste and vitellogen produced by fathead minnows will be used as a biomarker for assessment of endocrine disruption. Solid wastes from construction and demolition activities are generally placed on sites and in landfills that are not lined or otherwise designed or constructed to prevent or restrain leachate from contaminating soil and groundwater. Results of this research are likely to provide knowledge that can be used to determine the appropriate placement and engineering design of landfills to avoid pollution of soil, surface and groundwater by landfill leachates. The work is also expected to validate short-term assays that can be used to rapidly detect the potential a solid waste has for toxicity, endocrine disruption and other deleterious environmental effects on environmental resources.***