Establishment of a Fermentation Facility

请拨资金用于在南卡罗来纳大学生物研究和技术研究所建立完整细胞培养设施的发酵蛋白所需的仪器。这个设施将取代不能使用的28个L发酵罐，并将为大学社区提供广泛的发酵服务。用户组包括化学和生物科学系以及药学院和医学院的教职员工。该仪器支持的项目范围包括：(1)血红素加氧酶的结构和功能的研究；(2)胸苷合成酶的机制研究；(3)哺乳动物基因表达的研究；(4)胸苷合成酶和氟嘧啶抗性的研究；(5)基质囊泡膜联蛋白的结构功能研究；(6)用硒作为生物大分子结构/功能的探针；(7)细菌功能群的生理、生态和进化研究；(8)煤的溶解作用的遗传学研究；(9)弧菌基因调控机制的研究；(10)大鼠精子细胞转移蛋白2的结构研究；(11)抗肿瘤药物阿奇霉素A和B与DNA的相互作用；(12)烯醇化酶的机制研究；(13)枯草杆菌的细胞分裂基因产物；(14)人乳头瘤病毒在人类细胞转化中的作用；(15)假尿苷在mRNA剪接辅助因子中的形成和作用；(16)重组人重组αB/D杂交型干扰素治疗肝炎和乳头瘤病毒感染的临床前评估；和(17)整合素及其与细胞外基质的相互作用。

Funds are requested for instrumentation necessary to establish the fermentation protein of a complete Cell Culture Facility in the Institute for Biological Research and Technology at the University of South Carolina. This facility will replace an unusable 28 L fermenter, and will provide a wide range of fermentation services to the University community. The User Group includes faculty from the Department of Chemistry and Biological Sciences, as well as the College of Pharmacy and School of Medicine. The scope of projects to be supported by this instrumentation includes: (1) studies on the structure and function of hemeiron oxygenase enzymes; (2) mechanistic studies on thymidylate synthase; (3) studies on mammalian gene expression; (4) work on thymidylate synthase and fluoropyrimidine resistance; (5) structure function studies on matrix vesicle annexins; (6) using selenium as a probe of structure/function of biomacromolecules; (7) examining physiology, ecology, and evolution of bacterial functional group; (8) studies of the genetics of coal solubiolization; (9) mechanism of gene regulation in Caulobacter; (10) structural studies of rat spermatid transition protein 2; (11) examining the interaction of antitumor agents azinomycin A and B with DNA; (12) mechanistic studies of enolase; (13) cell division gene products of Bacillus subtilis; (14) role of human papillomaviruses in the transformation of human cells; (15) formation and role of pseudouridine in mRNA splicing cofactors; (16) preclinical evaluation of a human recombinant alph B/D hybrid interferon in therapy of hepatitis and papillomavirus infections; and (17) integrins and their interaction with the extracellular matrix.