New Brunswick 100 Liter Fermentation Facility in Memphis

新不伦瑞克省孟菲斯 100 升发酵设施

• 批准号: 6733478
• 负责人: RICHARD W KRIWACKI
• 金额: $ 19.93万
• 依托单位: ST. JUDE CHILDREN'S RESEARCH HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-01 至 2005-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6733478
• 关键词: biomedical equipment purchase; fermentation

DESCRIPTION (provided by investigator): We request funds to establish a large-scale (up to 100 liters) bacterial culture facility in Memphis, TN, to be located at St. Jude Children's Research Hospital (St. Jude). This facility will be available to serve a broad community of scientists in Memphis, including those at St. Jude, The University of Tennessee Health Sciences Center (U. Tennessee), St. Jude's partner in graduate education, and The University of Memphis (U. Memphis), a partner in many collaborative research projects. The purpose of this facility is to enable the large-scale production of proteins and other biomolecules for structural, biochemical and drug discovery studies. The proposed Fermentation Facility will impact a wide variety of NIH-funded biomedical research projects at the three institutions noted above, ranging from structural investigations into basic biological function and structure-based drug design, to the production of small molecules as intermediates for the synthesis of new therapeutics and high-throughput small-molecule screening. Overall, the Fermentation Facility will contribute to advancing research funded by more than one dozen R01, P01, U01, or R37 applications distributed between the three institutions. The principal contribution to ongoing research will be to enable large-scale production of biomolecule samples, especially proteins. In some cases, large-scale protein production is already under way but is performed in a highly time inefficient manner. Access to the proposed fermentor will allow scientists to focus much more of their time on biochemical and structural studies rather than on protein production. In other cases, production of certain proteins is currently out of reach due to low expression levels. Studies in this category will be made possible through access to the 100-liter fermentor. An additional benefit associated with creating this facility is the promotion of intra- and inter-institutional collaboration. While many of the participants here already collaborate, use of a shared Fermentation Facility will foster interactions between groups, especially at the level of graduate students and postdoctoral trainees who will actually perform fermentation experiments.

描述（由研究者提供）：我们请求资金在田纳西州孟菲斯的圣裘德儿童研究医院（St. Jude）建立一个大型（最多 100 升）细菌培养设施。该设施将为孟菲斯的广大科学家群体提供服务，包括圣裘德大学、田纳西大学健康科学中心（U. Tennessee）（圣裘德研究生教育合作伙伴）和孟菲斯大学（U. Memphis）（许多合作研究项目的合作伙伴）的科学家。该设施的目的是能够大规模生产蛋白质和其他生物分子，用于结构、生化和药物发现研究。 拟议的发酵设施将影响上述三个机构的 NIH 资助的各种生物医学研究项目，从基本生物功能的结构研究和基于结构的药物设计，到作为新疗法合成和高通量小分子筛选中间体的小分子的生产。总体而言，发酵设施将有助于推进由分布在三个机构之间的十多个 R01、P01、U01 或 R37 应用程序资助的研究。对正在进行的研究的主要贡献将是实现生物分子样品（尤其是蛋白质）的大规模生产。在某些情况下，大规模蛋白质生产已经在进行中，但是以一种时间效率非常低的方式进行的。使用拟议的发酵罐将使科学家能够将更多时间集中在生化和结构研究上，而不是蛋白质生产上。在其他情况下，由于表达水平低，某些蛋白质的生产目前无法实现。此类研究将通过使用 100 升发酵罐来实现。创建该设施的另一个好处是促进机构内和机构间的合作。虽然这里的许多参与者已经合作，但使用共享发酵设施将促进小组之间的互动，特别是在将实际进行发酵实验的研究生和博士后实习生层面。