Multi-user fermentation facility upgrade

描述(由申请人提供)：本申请申请资金用于更新加州大学洛杉矶分校的发酵设施，购买三个最大容量为5升、10升和19.5升的贝克曼“生产”发酵罐，四个可实时监测GFP荧光的实验大小的发酵罐/恒化器，以及一个细胞破碎器/均质器。该仪器将被NIH资助的研究人员用来培养各种生物，用于研究细胞生物学功能，并提纯蛋白质和蛋白质复合体，用于结构/功能研究。目前的设施有四个陈旧和不起作用的发酵罐。两个功能性发酵罐位于加州大学洛杉矶分校的校园里，但它们被安置在单独的实验室里。取而代之的是，大多数团体在多层摇壶中使用烧瓶。因此，生长微生物的实验室在实现最佳培养环境方面的能力是有限的，而纯化重组蛋白的研究人员在培养许多升细胞以获得足够数量的纯化蛋白方面面临着重大的后勤挑战。此外，改变生长条件的简单实验很难使用摇瓶系统进行调节，而且需要精确控制培养基的实验也是不可能的。 该发酵设施为加州大学洛杉矶分校NIH资助的研究人员提供服务，并集中安置在博耶霍尔。此外，加州大学洛杉矶分校美国能源部基因组和蛋白质组学研究所的蛋白质表达设施就在隔壁，有精通基本发酵技术的人员。大多数将使用发酵罐的小组已经熟悉发酵，但我们将任命一个由5名教职员工组成的咨询委员会来监督发酵罐的使用。发酵设施将由蛋白质表达设施的主任管理，他将每天监督发酵罐，操作发酵罐，根据需要提供指导，或将个人引导到咨询委员会的知识渊博的成员那里，以满足特殊需求。 发生的运营成本通常将在充值的基础上支付。此外，总理还为一项新的生物科学核心设施赠款计划提供了资金，以加强现有的核心和开发新的核心。该计划将用于增加新技术，包括服务合同，以及发酵设施的整体改进。如果资金得不到保障，充值机制将弥补必要的成本。 公共卫生相关性：为了了解宿主和病原体的相互作用，分析微生物如何在其环境中生长，并剖析细胞中的蛋白质途径，拥有一个最新的发酵系统是必不可少的。此外，大量与人类疾病有关的蛋白质的高效生产将有助于结构/结晶学研究。发酵系统将使加州大学洛杉矶分校由NIH资助的研究人员能够有效地进行这些类型的实验，这些实验影响到健康和福祉的许多方面。

DESCRIPTION (provided by applicant): This application requests funds to update the UCLA fermentation facility with the purchase of three new Beckman "production" fermentors with maximum capacities of 5, 10, and 19.5 liters, four experimental-sized fermentors/chemostats that can monitor GFP fluorescence in real-time, and a cell disrupter/homogenizer. The instrumentation will be used by the NIH-funded researchers to grow a variety of organisms for studying cell biology functions and purifying proteins and protein complexes for structure/function studies. The current facility has four fermentors that are antiquated and non-functional. Two functional fermentors are located on the UCLA campus but they are housed in individual laboratories. Instead, most groups use flasks in multi-tier shakers. As a result, labs growing microbes are limited in their ability to achieve optimal culture environments, and investigators purifying recombinant proteins are faced with significant logistic challenges in growing many liters of cells to obtain adequate quantities of purified proteins. Also, simple experiments to alter the growth conditions are difficult to regulate using a shake-flask system, and experiments requiring precise control of the culture medium are not possible. The fermentation facility serves the NIH-funded researchers at UCLA and is housed centrally in Boyer Hall. Also, the Protein Expression Facility in the UCLA-DOE Institute for Genomics and Proteomics is located next door and has individuals who are knowledgeable in basic fermentation techniques. Most groups that will use the fermentors are already familiar with fermentation, but we will appoint an Advisory Committee of 5 faculty members to oversee use of the fermentors. The fermentation facility will be run by the director of the Protein Expression Facility, who will oversee the fermentors on a daily basis, operating the fermentors, providing instruction as necessary, or directing the individual to a knowledgeable member of the advisory committee for specialized needs. Running costs that are incurred will generally be covered on a recharge basis. Additionally, the Chancellor has provided funds for a new Biosciences Core Facilities Grants Program to enhance existing cores and develop new ones. This program will be used for the addition of new technologies, covering service contracts, and overall improvements in the fermentation facility. If funds are not secured, the recharge mechanism will cover necessary costs. Public Health Relevance: To understand host-pathogen interactions, to analyze how microbes grow in their environment, and to dissect protein pathways in the cell, it is essential to have an updated fermentation system. Additionally, the efficient production of large quantities of proteins involved in human disease will aid in structural/crystallographic studies. The fermentation system will give the NIH-funded researchers at UCLA the ability to effectively conduct these types of experiments that impact many aspects of health and well-being.