A Fermentation and Cell Collection Facility at the University of Michigan

摘要DBI-9970145要求提供资金以购买用于细胞生长和收获的设备。 该设备将由密歇根大学中心校区的10个研究小组使用。将利用该设备的项目涉及生物化学和生物物理学的各个方面，但主要集中在机械酶学和蛋白质结构/功能研究。 将购买两个发酵罐。 小型发酵罐将用于制备同位素标记的蛋白质，而大型发酵罐将用于需要大量生长的项目，通常用于光谱实验。 大多数项目将受益于这两种发酵罐。 此外，还将购买一台连续流离心机和一台法式压榨机，用于分离和裂解细胞。 这种设备是迫切需要的，目前还没有。

AbstractDBI-9970145Funds are requested to purchase equipment that will be used for cell growth and harvesting. This equipment will be used by 10 research groups on the central campus of the University of Michigan. The projects that will utilize this equipment address diverse aspects of biochemistry and biophysics, but are primarily focused on mechanistic enzymology and protein structure/function studies. Two fermentors will be purchased. A small fermentor will be used for preparing isotopically labelled proteins while a large fermentor will be used by projects that require large volume growths, typically for spectroscopic experiments. Most of the projects will benefit from both fermentors. In addition, a continuous flow centrifuge and a French press will be purchased for isolation and lysing cells. This equipment is urgently needed and not presently available.