RUI: Signal Coupling During Endocytosis in Amoeba Proteus

RUI：变形虫胞吞过程中的信号耦合

• 批准号: 9118411
• 负责人: Robert Prusch
• 金额: $ 20.77万
• 依托单位: Gonzaga University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-02-01 至 1995-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9118411&HistoricalAwards=false
• 关键词: RUI; Signal; Coupling; During; Endocytosis

The major objectives of this proposed research are to continue basic investigations into the mechanism of signal-coupling during the initiation of endocytosis in Amoeba proteus and to provide some undergraduate students with a realistic research experience. Endocytosis is a fundamental process which involves the uptake of material external to a cell by surface membrane infolding and vesiculation. Previous work from this laboratory examined the interaction of signal molecules with the cell surface and identified several possible components of the signal pathway associated with the onset of endocytosis. These include a G- protein, cyclic AMP and calcium. A G-protein in the amoeba membrane was identified by ADP-ribosylation of a membrane protein(s) in the presence of cholera and pertussis toxin. Initiation of both phagocytosis and pinocytosis elicit rapid transient oscillations in cytoplasmic cAMP levels and cytoplasmic Ca increases transiently in stimulated cells. This proposal will attempt to further characterizethe components of the signal pathway and try to differentiate between the signal processes occurring during both pinocytosis and phagocytosis. The ameba represents a particularly useful model system in the study of endocytosis and it is expected that this study will increase our insight into the mechanism of endocytosis in this and other systems.

这项拟议研究的主要目标是继续 信号耦合机制的基本研究 变形变形虫内吞作用起始，并提供一些 本科生与现实的研究经验。 内吞作用是一个基本的过程，它涉及摄取 通过表面膜内折叠而位于细胞外部的物质， 囊泡形成 该实验室以前的工作检查了 信号分子与细胞表面的相互作用， 确定了信号通路的几个可能的组成部分 与内吞作用的发生有关。 其中包括G- 蛋白质环磷酸腺苷和钙 变形虫中的一种G蛋白 通过膜的ADP-核糖基化鉴定膜 在霍乱和百日咳毒素存在下的蛋白质。 吞噬作用和胞饮作用的启动引起快速的 细胞质cAMP水平和细胞质cAMP水平的瞬时振荡 钙在刺激的细胞中瞬时增加。 本提案将试图进一步描述组件的特性 并尝试区分信号 在胞饮作用和吞噬作用期间发生的过程。 的 阿米巴是研究中特别有用的模型系统。 内吞作用，预计这项研究将增加我们的 深入了解内吞作用的机制，在这个和其他 系统.