Fluorescence Microscopy for Cell Biology Laboratory

细胞生物学实验室荧光显微镜

• 批准号: 9350778
• 负责人: Robert Prusch
• 金额: $ 2.91万
• 依托单位: Gonzaga University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 1996-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9350778&HistoricalAwards=false
• 关键词: Fluorescence; Microscopy; Cell; Biology; Laboratory

9350778 Prusch The Biology Department at Gonzaga University is attempting to both increase and strengthen the laboratory component of a newly-revised and expanded Cell Biology course and to increase research opportunities for undergraduate majors. The former upper division one semester Cell and Molecular Biology course has been expanded to two semesters and is now directed at sophomore level biology majors. The laboratory component of this expanded Cell Biology course will introduce students to a variety of techniques used in cell biology as well as reinforce concepts introduced in the lecture portion of the course. One of the most basic as well as important techniques used routinely in cell biology is fluorescence microscopy. Fluorescence microscopy can be used to follow a wide variety of events in living cells in real time, as well as the distribution of specific substances in fixed cells. For example, students will be able to study endocytosis in living cells from the initial interaction of a signal with receptors on the cell surface to endosome or phagosome formation. Cytoskeletal rearrangements in response to environmental stimuli and localized biochemical events in living cells will be studied. Acquisition of fluorescence microscopes will give biology students new experiences in their laboratories and greatly broaden the opportunities for the use of modern technology in their own research projects. ***

9350778 Prusch贡扎加大学生物系正在试图增加和加强新修订和扩展的细胞生物学课程的实验室部分，并增加本科专业的研究机会。前上师一学期细胞和分子生物学课程已扩大到两个学期，现在是针对大二水平的生物学专业。本扩展细胞生物学课程的实验室部分将向学生介绍细胞生物学中使用的各种技术，并加强课程讲座部分中介绍的概念。荧光显微镜是细胞生物学中常用的最基本也是最重要的技术之一。 荧光显微镜可用于真实的跟踪活细胞中的各种事件，以及固定细胞中特定物质的分布。例如，学生将能够研究活细胞中的内吞作用，从信号与细胞表面受体的最初相互作用到内体或吞噬体的形成。 将研究细胞骨架重排对环境刺激和活细胞中局部生化事件的反应。购买荧光显微镜将为生物学学生在实验室提供新的体验，并大大拓宽在自己的研究项目中使用现代技术的机会。 ***