RUI: Glutathione S-transferase Enzyme(s) and the Conjugation of Plant Phenolics

RUI：谷胱甘肽 S-转移酶和植物酚类化合物的结合

• 批准号: 9119296
• 负责人: John Dean
• 金额: $ 20万
• 依托单位: DePaul University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-03-01 至 1996-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9119296&HistoricalAwards=false
• 关键词: RUI; Glutathione; transferase; Enzyme; Conjugation

Many plants are known to increase the intracellular levels of phenolic compounds when they are challenged by a wide variety of stressful conditions including predation. Many phenolic compounds are toxic to a wide variety of biotic agents. In order for plants to use this defense mechanism, the species must be able to tolerate the increased level of the phenolic. Plants reduce the autotoxicity of phenolics by conjugating them to other cellular constituents. It has been recently noted that plants may conjugate some phenolics to glutathione through the action of a glutathione S-transferase. Preliminary evidence indicates that the activity of the enzyme may be regulated by phenolic levels. The objectives of this project are to: 1) characterize the glutathione S- transferase enzyme; 2) determine if plants synthesize glutathione conjugates of phenolics in vivo; and 3) determine if there is a correlation between stress and glutathione S-transferase activity. An understanding of the role of glutathione in phenolic conjugation will aid in the understanding of how plants utilize phenolics during times of stress and may provide insight into the endogenous function of plants GST enzymes.

已知许多植物增加细胞内的 酚类化合物，当它们受到各种各样的挑战时， 包括捕食的压力条件。 许多酚类化合物 对很多生物体都是有毒的 为了让植物 为了使用这种防御机制，物种必须能够忍受 酚类物质含量的增加 植物减少了 通过将酚类化合物与其它细胞结合， 选民。 最近有人注意到，植物可以结合 一些酚类物质通过谷胱甘肽的作用转化为谷胱甘肽 S-转移酶 初步证据显示， 酶的活性可能受酚类物质水平的调节。 目标 本项目的目的是：1）表征谷胱甘肽S- 转移酶; 2）确定植物是否合成谷胱甘肽 酚类化合物在体内的缀合物;和3）确定是否存在 应激与谷胱甘肽S-转移酶活性之间的相关性。 谷胱甘肽在酚类结合中的作用 将有助于了解植物如何利用酚类物质 在压力时期，并可能提供对内源性 植物GST酶的功能。