The Ecology and Population Biology of the Fungus Aureobasidium pullulans on Leaf Surfaces

生长在树叶表面的真菌的生命周期 在叶片上的阶段，在大气中的运输阶段，以及 增长 在同一寄主或同一物种寄主的另一片叶子上， 不同 宿主物种，或在一些其他基质上。 基本上没有什么是 定量地了解这些真菌在原地的人口统计学。 本研究的最终目标是为 苹果叶片上的一种类似酵母的真菌，出芽短梗霉 表面。 为了给人口统计学研究提供基础， 发展追踪所有附生成员的分子方法 的 助理提出了普鲁兰菌的原位种群。 二是 区分引入的背景群体，（GUS） 基因 来自大肠coli将被安装到A.从而允许 真菌的检测显色或荧光 原地。 活细胞与死细胞将由一个重要的 染色法 该系统将根据以下标准进行评估和建模 控制和不控制的系统，然后用来测试 假设“来源”地区（增长-移民; 生长死亡+迁移）。叶子上的普鲁兰多糖. 在树冠的不同位置。 该项目 对基本生态学（社区进程和 结构）和用于植物病原体的生物防治。

Fungi that grow on leaf surfaces alternate between a life cycle phase on the leaf, a transport phase in the atmosphere, and growth on another leaf of the same host or host of species, on a different host species, or on some other substrate. Essentially nothing is known quantitatively about the demography of these fungi in situ. The ultimate goal of this research is to produce a model for the yeast-like fungus, Aureobasidium pullulans, on apple leaf surfaces. To provide the foundation for the demographic study, the further development of molecular methods to track all epiphytic members of the A. pullulans population in situ is proposed. Second, to discriminate introduced from background populations, the (GUS) gene from E. coli will be installed into A. pullulans thereby allowing the detection of fungus chromogenically or fluorogenically in situ. Live versus dead cells will be confirmed independently by a vital staining method. The system will be evaluated and modelled under controlled and uncontrolled systems and then used to test the hypothesis that "source" areas (growthimmigration; and growthdeath + emigration) exist for A. pullulans on leaves at different positions within the tree canopy. The project has implications for both basic ecology (community processes and structure) and for the biological control of plant pathogens.