High-throughout fluorescent imaging system: From cell biology to population ecology

高通量荧光成像系统：从细胞生物学到群体生态学

• 批准号: RTI-2018-00282
• 负责人: Heyland, Andreas
• 金额: $ 10.91万
• 依托单位: University of Guelph
• 依托单位国家: 加拿大
• 项目类别: Research Tools and Instruments
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=642175
• 关键词: throughout; fluorescent; imaging; system; cell

We are applying for the urgent replacement of a high-end Nikon Ti image acquisition system with necessary upgrades. Two highly productive research programs and at least 7 laboratories with multiple students depend on the proposed equipment and have been unable to complete essential high-throughput multi-dimensional assays due to A) limitations with the existing equipment and B) mechanical failures of the existing equipment due to aging. We therefore propose to purchase a highly customizable high content imaging platform from Nikon, designed to serve the immediate needs of two laboratories in the Department of Integrative Biology at the University of Guelph and 7 laboratories in the College of Biological Sciences. Research in these laboratories covers several levels of organization (molecules, cells, embryos and larvae) and approaches (population ecology, cell biology, molecular biology and developmental biology) and there is an urgent need for all laboratories for high throughput acquisitions. Specifically, the applicant’s ongoing research programs and newly established collaborations with industry and other laboratories require the urgent implementation of high content acquisition systems, a task that cannot be handled by the existing microscope. Since the previous unsuccessful submission of this application last year, the acquisition of the new microscope system has become even more urgent due to repeated technical failures of the existing system and the inability to implement novel high-throughput protocols required for ongoing projects. The core imaging facility at the University of Guelph is not setup to accommodate proposed operating procedures for automated workflows as their microscopy systems generally consist of highly specialized confocal microscopes without high throughput capacity. Furthermore the existing systems can only handle the needs of a few labs until capacity is reached. Without the proposed infrastructure important momentum and opportunity will be lost and ongoing work in the Heyland and Fryxell labs will be stalled until new infrastructure is available.

我们正在申请紧急更换高端尼康Ti图像采集系统，并进行必要的升级。两个高生产力的研究项目和至少7个有多名学生的实验室依赖于所提出的设备，并且由于A）现有设备的限制和B）现有设备由于老化导致的机械故障而无法完成必要的高通量多维测定。因此，我们建议从尼康购买高度可定制的高内容成像平台，旨在满足圭尔夫大学综合生物学系两个实验室和生物科学学院7个实验室的迫切需求。这些实验室的研究涵盖了几个层次的组织（分子，细胞，胚胎和幼虫）和方法（种群生态学，细胞生物学，分子生物学和发育生物学），迫切需要所有实验室进行高通量收购。具体而言，申请人正在进行的研究计划以及与工业和其他实验室新建立的合作需要紧急实施高内容采集系统，这是现有显微镜无法处理的任务。自去年提交该申请失败以来，由于现有系统一再出现技术故障，无法实施正在进行的项目所需的新型高通量协议，因此新显微镜系统的收购变得更加紧迫。圭尔夫大学的核心成像设施并没有设置为适应自动化工作流程的拟议操作程序，因为他们的显微镜系统通常由高度专业化的共焦显微镜组成，没有高通量。此外，现有的系统只能满足少数实验室的需求，直到达到容量。如果没有拟议的基础设施，将失去重要的动力和机会，在新的基础设施可用之前，Heyland和Fryxell实验室正在进行的工作将停滞不前。