Molecular Phylogeny of the Poeciliidae (Pisces, Teleostei)

Poeciliidae（双鱼座、硬骨鱼）的分子系统发育

• 批准号: 9119867
• 负责人: A. Meyer
• 金额: $ 23万
• 依托单位: SUNY at Stony Brook
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-04-01 至 1996-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9119867&HistoricalAwards=false
• 关键词: Molecular; Phylogeny; Poeciliidae; Pisces; Teleostei

Poeciliids (Pisces, Poeciliidae), the guppies, swordtails, and mollies, are a family of small fishes that are frequently used as a model system in many biological studies. Research topics range from zoogeography, cancer, mating systems, natural and sexual selection, comparative ecology, to conservation biology. Despite their widespread use, very little is known about the phylogenetic or genealogical relationships of poeciliids. The aim of this project by Dr. Axel Meyer at SUNY-Stonybrook is to establish such a phylogeny for the poeciliids using mitochondrial and nuclear DNA sequence data as measures of mutational differences between species. The complete sequence of the mitochondrial gene cytochrome b (about 1100 base pairs) and partial sequences of a nuclear gene, the proto-oncogene X-src (about 520 base pairs), will be obtained from several representatives of the species and genera in this family. The use of both mitochondrial and nuclear sequence data permits the inference of phylogenetic relationship across broad taxonomic levels, from species to family, and also provides independent checking of the resulting phylogenetic estimates. These estimates will then permit testing of vicariance models of biogeography and other aspects of evolutionary diversification in this group of fishes.

鲤科（双鱼座，鲤科），孔雀鱼，剑尾鱼和摩利鱼，是一种小型鱼类，经常被用作许多生物学研究的模型系统。研究课题包括动物地理学、癌症、交配系统、自然和性选择、比较生态学和保护生物学。尽管它们被广泛使用，但对水蛭的系统发育或系谱关系知之甚少。该项目由纽约州立大学石溪分校的阿克塞尔·迈耶博士负责，目的是利用线粒体和核DNA序列数据作为物种间突变差异的衡量标准，为拟水龙建立这样一个系统发育。线粒体基因细胞色素b的完整序列（约1100个碱基对）和核基因原癌基因X-src的部分序列（约520个碱基对）将从该科的几个代表物种和属中获得。线粒体和核序列数据的使用允许在广泛的分类水平上推断系统发育关系，从物种到科，并且还提供了对系统发育估计的独立检查。这些估计将允许测试生物地理学的变异模型和这类鱼类进化多样化的其他方面。