Infrared and Ultraviolet Matrix-Assisted Laser Desorption in a Quadrupole Ion Trap

该项目由分析和表面化学 程序，检查离子分子化学在四极离子 陷阱 这项研究的目的是了解 离子分子化学的原理， 大有机分子的光谱分析，最终 碳水化合物抗生素 初步工作将 专注于相关分子的激光解吸， 四极离子阱 对后续的替代效应 离子阱中的双分子离子-分子反应将是 为了利用这些反应， 大型复杂有机分子的结构鉴定。 %%% 碳水化合物是生物学中极其重要的分子 系统，但其结构的确定是非常重要的 困难，特别是因为他们通常不能 进行X射线结构研究。 测定 通过质谱分析结构也很困难，因为许多 有趣的碳水化合物很难蒸发， 质谱法是气相技术。本研究 项目激光解吸方法将用于放置 气体中的碳水化合物和其他大的有机分子 相和受控离子分子反应化学将是 用来将大分子按质量切割成碎片 光谱分析，如结构分析所需。

This project, supported by the Analytical and Surface Chemistry Program, examines ion-molecule chemistry in the quadrupole ion trap. The goal of the research is to develop an understanding of ion-molecule chemistry as it applies to the mass spectrometric analysis of large organic molecules, eventually carbohydrate-containing antibiotics. Initial work will concentrate on the laser desorption of relevant molecules into the quadrupole ion trap. Substituent effects on the subsequent bimolecular ion-molecule reactions in the ion trap will be explored, with a view to exploiting these reactions for structural identification of large, complex organic molecules. %%% Carbohydrates are extremely important molecules in biological systems, but the determination of their structures is very difficult, especially because they usually cannot be crystallized for X-ray structural studies. Determination of structures by mass spectrometry is also difficult, since many interesting carbohydrates are very difficult to vaporize, and mass spectrometry is a gas phase technique. In this research project laser desorption methods will be used to place carbohydrates and other large organic molecules in the gas phase, and controlled ion-molecule reaction chemistry will be used to cut the large molecules into fragments by mass spectrometry, as required for structural analysis.