Photodissociation and Ion Trap Mass Spectrometry

在这个奖项中，由化学系的化学测量和成像计划资助，Brodbelt教授和她的学生将结合化学衍生方法开发光解离，该方法将发色团和/或电荷位点添加到分子中，以修改离子碎片模式。质谱已成为生物分子结构表征的最通用的方法之一，并且增强质谱策略的选择性仍然是优先考虑的。这项工作将遵循一个主题，旨在通过使用离子活化方法，结合有针对性的选择性的生物分子的结构表征，同时也建立了一个框架，了解特定位点的生色团对离子活化的影响。主要目标包括评估电荷状态对解离效率的影响，连接发色团以提高解离效率，将选择性可裂解的键并入分子中，以及开发用于蛋白质高通量分析的光解离。除了这项建议的重要科学目标外，未来科学家的教育也是一个具有长期影响的重要成果。需要开发更好的分析工具，包括拟议工作中追求的质谱策略，以阐明负责许多生物功能的分子的结构，例如对抗病毒和心脏肌肉收缩的抗体。从拟议的工作中产生的预期利益包括：1）在分析化学和生物质谱领域培训和教育未来的科学家，2）开发基于激光的创新策略，用于生物分子的结构表征，如肽，蛋白质，碳水化合物和核酸。

In this award, funded by the Chemical Measurement and Imaging Program of the Chemistry Division, Professor Brodbelt and her students will develop photodissociation in conjunction with chemical derivatization methods that add chromophores and/or charge sites to molecules in order to modify ion fragmentation patterns. Mass spectrometry has become one of the most versatile methods for structural characterization of biological molecules, and enhancing the selectivity of mass spectrometric strategies remains a priority. This work will follow a theme aimed at the structural characterization of biological molecules by using ion activation methods that incorporate targeted selectivity, while also building a framework for understanding the impact of site-specific chromophores on ion activation. The primary goals include the evaluation of charge state on dissociation efficiency, the attachment of chromophores to enhance dissociation efficiencies, the incorporation of selectively cleavable bonds into molecules, and the development of photodissociation for high throughput analysis of proteins. In addition to the important scientific goals of this proposal, the education of future scientists is an equally significant outcome with long-term impact. The development of better analytical tools, including the mass spectrometric strategies pursued in the proposed work, is needed to elucidate the structures of molecules responsible for many biological functions, such as antibodies that fight viruses and muscle contraction in the heart. The anticipated benefits arising from the proposed work include: 1) the training and education of future scientists in the area of analytical chemistry and biological mass spectrometry, and 2) the development of innovative laser-based strategies for structural characterization of biological molecules such as peptides, proteins, carbohydrates, and nucleic acids.