Photodissociation in a Quadrupole Ion Trap

四极离子阱中的光解离

• 批准号: 0315337
• 负责人: Jennifer Brodbelt
• 金额: --
• 依托单位: University of Texas at Austin
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-15 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0315337&HistoricalAwards=false
• 关键词: Photodissociation; Quadrupole; Ion; Trap

Professor Jennifer Brodbelt of the University of Texas Austin will work with Professor Alan Marchand of the University of North Texas on a project in bioanalytical chemistry supported by the Analytical and Surface Chemistry Program. This project aims to study and improve photodissociation methods in the quadrupole ion trap mass spectrometer for analysis of peptides and proteins. The approach will be to complex the analyte species with metal ions and/or organic ligands that will more effectively absorb photons from the photodissociation laser and thus transfer sufficient energy to the analyte for fragmentation. Optimization of photodissociation will also be explored for high-performance liquid chromatography (HPLC) analysis. The proposed work combines the expertise of Professor Broadbelt in mass spectrometry (MS) and ion chemistry, and Professor Marchand in synthesis and macrocyclic chemistry. Prof. Brodbelt's group has published extensively on the two building blocks of the proposed research-first, photodissociation of organic and biological compounds, and second, enhancement of ion activation (collision-induced dissociation) through complex formation. The present work brings these two lines of research together, and extends them to potential applications involving HPLC-MS. HPLC-MS analysis of peptides and proteins is an important tool for proteomics, but would benefit from the improved ion activation techniques explored here. The collaboration with Prof. Marchand, whose group will synthesize novel ligands for complexation, allows cross training that will be valuable for students in both the analytical chemistry and organic chemistry groups.

德克萨斯大学奥斯汀分校的詹妮弗·布罗德贝尔特（Jennifer Brodbelt）教授将与北德克萨斯大学的艾伦·马尔坎德（Alan Marchand）教授合作，由分析和地表化学计划支持的生物分析化学项目。 该项目旨在研究和改善四极离子陷阱质谱仪中的光解离方法，以分析肽和蛋白质。该方法将是将分析物物种与金属离子和/或有机配体复杂化，这些金属离子和/或有机配体将更有效地从光解离激光器中吸收光子，从而将足够的能量传递到分析物以进行碎片化。还将探索光解离的优化，以进行高性能液相色谱（HPLC）分析。拟议的工作结合了Broadbelt教授在质谱（MS）和离子化学方面的专业知识，以及Marchand教授在合成和大环化学方面的专业知识。 Brodbelt教授的小组已广泛发表了提出的研究优先的两个构建基块，有机和生物化合物的光电分离，其次，通过复杂形成增强了离子激活（碰撞诱导的解离）的增强。目前的工作将这两条研究汇总在一起，并将其扩展到涉及HPLC-MS的潜在应用。肽和蛋白质的HPLC-MS分析是蛋白质组学的重要工具，但将从此处探索的改进的离子激活技术中受益。与Marchand教授的合作，其小组将合成新颖的配体进行络合，允许交叉训练，这对于分析化学和有机化学组的学生都很有价值。