Ultraviolet Photodissociation Mass Spectrometry

紫外光解离质谱法

基本信息

批准号：
2203602
负责人：
Jennifer Brodbelt
金额：
$ 63万
依托单位：
University of Texas at Austin
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2022
资助国家：
美国
起止时间：
2022-06-01 至 2025-05-31
项目状态：
未结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2203602&HistoricalAwards=false
关键词：
Ultraviolet Photodissociation Mass Spectrometry

项目摘要

With support from the Chemical Measurement and Imaging program in the Division of Chemistry, Professor Jenny Brodbelt and her group at the University of Texas at Austin are developing innovative methods to characterize proteins using mass spectrometry (MS). Proteins in cells participate in a vast array of functions necessary to sustain life, and the physiological functions and activities of proteins are modulated by structural modifications. Dr. Brodbelt and her group develop new high performance methods that combine MS and both gas-phase separations and reactions to examine proteins and pinpoint the modifications in molecular detail. In particular, the Brodbelt group uses high energy lasers to shatter proteins to create diagnostic fragmentation patterns that reveal structures and modifications. The comprehensive plan engages students at multiple levels in collaborative, interdisciplinary research and provides professional training for the transition to scientific careers. Analysis of intact proteins remains one of the frontiers of proteomics, and the ability to characterize intact proteins offers one of the best means to map combinatorial modifications that play critical roles in protein structure and function. A central hallmark of the Brodbelt group over the past decade is the development and application of ultraviolet photodissociation (UVPD) for analysis of biological molecules. This project emphasizes the development and applications of UVPD for analysis of intact proteins. The main aims integrate dynamic ion manipulation strategies utilizing proton transfer reactions, gas-phase fractionation, and charge detection measurement methods to amplify the information content of MS/MS spectra of intact proteins. UVPD is used to characterize isomers separated by ion mobility via a modular drift cell, and collision cross-sections of proteins are measured. These innovative methods are applied to decipher patterns of post-translational modifications of proteins and extend the mass range of top-down proteomics. The training and mentoring activities forge partnerships with graduate and undergraduate students to encourage their professional development into future leaders in academic and industrial sectors.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在化学分区的化学测量和成像计划的支持下，珍妮·布罗德贝尔特（Jenny Brodbelt）教授及其在奥斯汀分校的小组正在开发创新的方法，以使用质谱法（MS）来表征蛋白质。细胞中的蛋白质参与维持生命所需的大量功能，蛋白质的生理功能和活性受结构修饰的调节。 Brodbelt博士和她的小组开发了新的高性能方法，该方法将MS和气相分离和反应结合在一起，以检查蛋白质并以分子细节来查明修饰。特别是，Brodbelt组使用高能量激光器来破碎蛋白质，以创建诊断片段化模式，以揭示结构和修饰。全面的计划使学生参与多个级别的协作，跨学科研究，并为过渡到科学职业提供专业培训。完整蛋白质的分析仍然是蛋白质组学的前沿之一，并且表征完整蛋白质的能力是映射组合修饰的最佳方法之一，这些方法在蛋白质结构和功能中起着至关重要的作用。在过去十年中，Brodbelt群的核心标志是紫外线光解离（UVPD）用于分析生物分子的发展和应用。该项目强调了UVPD用于分析完整蛋白质的开发和应用。主要目的是整合利用质子转移反应，气相分级和电荷检测测量方法的动态离子操纵策略，以扩大完整蛋白质的MS/MS光谱的信息含量。 UVPD用于表征通过模块漂移细胞通过离子迁移率分离的异构体，并测量蛋白质的碰撞横截面。这些创新方法应用于蛋白质翻译后修饰的解密模式，并扩展了自上而下的蛋白质组学的质量范围。培训和指导活动与研究生和本科生建立了合作伙伴关系，以鼓励他们的专业发展成为学术和工业领域的未来领导者。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子优点和更广泛影响的评估标准通过评估来获得支持的。