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Ultraviolet Photodissociation Mass Spectrometry

紫外光解离质谱法

基本信息

批准号：
2203602
负责人：
Jennifer Brodbelt
金额：
$ 63万
依托单位：
University of Texas at Austin
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2022
资助国家：
美国
起止时间：
2022-06-01 至 2025-05-31
项目状态：
未结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2203602&HistoricalAwards=false
关键词：
Ultraviolet Photodissociation Mass Spectrometry

项目摘要

With support from the Chemical Measurement and Imaging program in the Division of Chemistry, Professor Jenny Brodbelt and her group at the University of Texas at Austin are developing innovative methods to characterize proteins using mass spectrometry (MS). Proteins in cells participate in a vast array of functions necessary to sustain life, and the physiological functions and activities of proteins are modulated by structural modifications. Dr. Brodbelt and her group develop new high performance methods that combine MS and both gas-phase separations and reactions to examine proteins and pinpoint the modifications in molecular detail. In particular, the Brodbelt group uses high energy lasers to shatter proteins to create diagnostic fragmentation patterns that reveal structures and modifications. The comprehensive plan engages students at multiple levels in collaborative, interdisciplinary research and provides professional training for the transition to scientific careers. Analysis of intact proteins remains one of the frontiers of proteomics, and the ability to characterize intact proteins offers one of the best means to map combinatorial modifications that play critical roles in protein structure and function. A central hallmark of the Brodbelt group over the past decade is the development and application of ultraviolet photodissociation (UVPD) for analysis of biological molecules. This project emphasizes the development and applications of UVPD for analysis of intact proteins. The main aims integrate dynamic ion manipulation strategies utilizing proton transfer reactions, gas-phase fractionation, and charge detection measurement methods to amplify the information content of MS/MS spectra of intact proteins. UVPD is used to characterize isomers separated by ion mobility via a modular drift cell, and collision cross-sections of proteins are measured. These innovative methods are applied to decipher patterns of post-translational modifications of proteins and extend the mass range of top-down proteomics. The training and mentoring activities forge partnerships with graduate and undergraduate students to encourage their professional development into future leaders in academic and industrial sectors.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在化学系化学测量和成像计划的支持下，德克萨斯大学奥斯汀分校的Jenny Brodbelt教授和她的团队正在开发使用质谱（MS）表征蛋白质的创新方法。细胞中的蛋白质参与维持生命所必需的大量功能，并且蛋白质的生理功能和活性通过结构修饰来调节。Brodbelt博士和她的团队开发了新的高性能方法，该方法将联合收割机MS与气相分离和反应相结合，以检查蛋白质并精确定位分子细节的修饰。特别是，Brodbelt小组使用高能激光粉碎蛋白质，以创建诊断碎片模式，揭示结构和修饰。该综合计划使学生在多个层次上进行协作，跨学科研究，并为过渡到科学职业提供专业培训。完整蛋白质的分析仍然是蛋白质组学的前沿之一，并且表征完整蛋白质的能力提供了绘制在蛋白质结构和功能中起关键作用的组合修饰的最佳手段之一。Brodbelt小组在过去十年中的一个中心标志是紫外光解离（UVPD）在生物分子分析中的发展和应用。本项目着重于UVPD在完整蛋白质分析中的发展和应用。主要目标集成动态离子操作策略，利用质子转移反应，气相分馏，和电荷检测测量方法，以放大完整蛋白质的MS/MS光谱的信息内容。 UVPD是用来表征异构体分离离子迁移率通过模块化的漂移池，和蛋白质的碰撞截面进行测量。这些创新的方法被应用于破译蛋白质翻译后修饰的模式，并扩展了自上而下的蛋白质组学的质量范围。培训和指导活动与研究生和本科生建立伙伴关系，鼓励他们的专业发展成为未来的学术和工业部门的领导者。该奖项反映了NSF的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。