权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Ultraviolet Photodissociation Mass Spectrometry

紫外光解离质谱法

基本信息

批准号：
2203602
负责人：
Jennifer Brodbelt
金额：
$ 63万
依托单位：
University of Texas at Austin
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2022
资助国家：
美国
起止时间：
2022-06-01 至 2025-05-31
项目状态：
未结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2203602&HistoricalAwards=false
关键词：
Ultraviolet Photodissociation Mass Spectrometry

项目摘要

With support from the Chemical Measurement and Imaging program in the Division of Chemistry, Professor Jenny Brodbelt and her group at the University of Texas at Austin are developing innovative methods to characterize proteins using mass spectrometry (MS). Proteins in cells participate in a vast array of functions necessary to sustain life, and the physiological functions and activities of proteins are modulated by structural modifications. Dr. Brodbelt and her group develop new high performance methods that combine MS and both gas-phase separations and reactions to examine proteins and pinpoint the modifications in molecular detail. In particular, the Brodbelt group uses high energy lasers to shatter proteins to create diagnostic fragmentation patterns that reveal structures and modifications. The comprehensive plan engages students at multiple levels in collaborative, interdisciplinary research and provides professional training for the transition to scientific careers. Analysis of intact proteins remains one of the frontiers of proteomics, and the ability to characterize intact proteins offers one of the best means to map combinatorial modifications that play critical roles in protein structure and function. A central hallmark of the Brodbelt group over the past decade is the development and application of ultraviolet photodissociation (UVPD) for analysis of biological molecules. This project emphasizes the development and applications of UVPD for analysis of intact proteins. The main aims integrate dynamic ion manipulation strategies utilizing proton transfer reactions, gas-phase fractionation, and charge detection measurement methods to amplify the information content of MS/MS spectra of intact proteins. UVPD is used to characterize isomers separated by ion mobility via a modular drift cell, and collision cross-sections of proteins are measured. These innovative methods are applied to decipher patterns of post-translational modifications of proteins and extend the mass range of top-down proteomics. The training and mentoring activities forge partnerships with graduate and undergraduate students to encourage their professional development into future leaders in academic and industrial sectors.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在化学系化学测量和成像项目的支持下，德克萨斯大学奥斯汀分校的 Jenny Brodbelt 教授和她的团队正在开发利用质谱 (MS) 表征蛋白质的创新方法。细胞中的蛋白质参与维持生命所需的大量功能，并且蛋白质的生理功能和活性通过结构修饰来调节。 Brodbelt 博士和她的团队开发了新的高性能方法，将 MS 与气相分离和反应相结合，以检查蛋白质并精确定位分子细节的修饰。特别是，布罗德贝尔特小组使用高能激光粉碎蛋白质，以创建可揭示结构和修饰的诊断碎片模式。该综合计划让多个层次的学生参与协作、跨学科研究，并为向科学职业的过渡提供专业培训。完整蛋白质的分析仍然是蛋白质组学的前沿之一，表征完整蛋白质的能力提供了绘制在蛋白质结构和功能中发挥关键作用的组合修饰的最佳方法之一。 Brodbelt 团队过去十年的核心标志是开发和应用紫外光解 (UVPD) 来分析生物分子。该项目强调 UVPD 在完整蛋白质分析中的开发和应用。主要目标是利用质子转移反应、气相分馏和电荷检测测量方法整合动态离子操纵策略，以放大完整蛋白质的 MS/MS 谱的信息内容。 UVPD 用于表征通过模块化漂移池的离子淌度分离的异构体，并测量蛋白质的碰撞截面。这些创新方法用于破译蛋白质翻译后修饰的模式，并扩展自上而下蛋白质组学的质量范围。培训和指导活动与研究生和本科生建立了合作伙伴关系，鼓励他们的专业发展成为学术和工业领域的未来领导者。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。