Ultraviolet Photodissociation Mass Spectrometry
紫外光解离质谱法
基本信息
- 批准号:2203602
- 负责人:
- 金额:$ 63万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-06-01 至 2025-05-31
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
With support from the Chemical Measurement and Imaging program in the Division of Chemistry, Professor Jenny Brodbelt and her group at the University of Texas at Austin are developing innovative methods to characterize proteins using mass spectrometry (MS). Proteins in cells participate in a vast array of functions necessary to sustain life, and the physiological functions and activities of proteins are modulated by structural modifications. Dr. Brodbelt and her group develop new high performance methods that combine MS and both gas-phase separations and reactions to examine proteins and pinpoint the modifications in molecular detail. In particular, the Brodbelt group uses high energy lasers to shatter proteins to create diagnostic fragmentation patterns that reveal structures and modifications. The comprehensive plan engages students at multiple levels in collaborative, interdisciplinary research and provides professional training for the transition to scientific careers. Analysis of intact proteins remains one of the frontiers of proteomics, and the ability to characterize intact proteins offers one of the best means to map combinatorial modifications that play critical roles in protein structure and function. A central hallmark of the Brodbelt group over the past decade is the development and application of ultraviolet photodissociation (UVPD) for analysis of biological molecules. This project emphasizes the development and applications of UVPD for analysis of intact proteins. The main aims integrate dynamic ion manipulation strategies utilizing proton transfer reactions, gas-phase fractionation, and charge detection measurement methods to amplify the information content of MS/MS spectra of intact proteins. UVPD is used to characterize isomers separated by ion mobility via a modular drift cell, and collision cross-sections of proteins are measured. These innovative methods are applied to decipher patterns of post-translational modifications of proteins and extend the mass range of top-down proteomics. The training and mentoring activities forge partnerships with graduate and undergraduate students to encourage their professional development into future leaders in academic and industrial sectors.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
在化学系化学测量和成像计划的支持下,德克萨斯大学奥斯汀分校的Jenny Brodbelt教授和她的团队正在开发使用质谱(MS)表征蛋白质的创新方法。 细胞中的蛋白质参与维持生命所必需的大量功能,并且蛋白质的生理功能和活性通过结构修饰来调节。Brodbelt博士和她的团队开发了新的高性能方法,该方法将联合收割机MS与气相分离和反应相结合,以检查蛋白质并精确定位分子细节的修饰。 特别是,Brodbelt小组使用高能激光粉碎蛋白质,以创建诊断碎片模式,揭示结构和修饰。 该综合计划使学生在多个层次上进行协作,跨学科研究,并为过渡到科学职业提供专业培训。 完整蛋白质的分析仍然是蛋白质组学的前沿之一,并且表征完整蛋白质的能力提供了绘制在蛋白质结构和功能中起关键作用的组合修饰的最佳手段之一。Brodbelt小组在过去十年中的一个中心标志是紫外光解离(UVPD)在生物分子分析中的发展和应用。本项目着重于UVPD在完整蛋白质分析中的发展和应用。主要目标集成动态离子操作策略,利用质子转移反应,气相分馏,和电荷检测测量方法,以放大完整蛋白质的MS/MS光谱的信息内容。 UVPD是用来表征异构体分离离子迁移率通过模块化的漂移池,和蛋白质的碰撞截面进行测量。 这些创新的方法被应用于破译蛋白质翻译后修饰的模式,并扩展了自上而下的蛋白质组学的质量范围。 培训和指导活动与研究生和本科生建立伙伴关系,鼓励他们的专业发展成为未来的学术和工业部门的领导者。该奖项反映了NSF的法定使命,并通过使用基金会的智力价值和更广泛的影响审查标准进行评估,被认为值得支持。
项目成果
期刊论文数量(15)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Enhancing Top-Down Analysis of Proteins by Combining Ultraviolet Photodissociation (UVPD), Proton-Transfer Charge Reduction (PTCR), and Gas-Phase Fractionation to Alleviate the Impact of Nondissociated Precursor Ions
- DOI:10.1021/jasms.3c00351
- 发表时间:2023-12-27
- 期刊:
- 影响因子:3.2
- 作者:Dunham,Sean D.;Brodbelt,Jennifer S.
- 通讯作者:Brodbelt,Jennifer S.
Enhanced Characterization of Histones Using 193 nm Ultraviolet Photodissociation and Proton Transfer Charge Reduction
使用 193 nm 紫外光解和质子转移电荷减少增强组蛋白的表征
- DOI:10.1021/acs.analchem.2c05765
- 发表时间:2023
- 期刊:
- 影响因子:7.4
- 作者:Walker, Jada N.;Lam, Raymond;Brodbelt, Jennifer S.
- 通讯作者:Brodbelt, Jennifer S.
Improving Ion Mobility Mass Spectrometry of Proteins through Tristate Gating and Optimization of Multiplexing Parameters
- DOI:10.1021/jasms.2c00274
- 发表时间:2022-12-05
- 期刊:
- 影响因子:3.2
- 作者:Butalewicz, Jamie P.;Sanders, James D.;Brodbelt, Jennifer S.
- 通讯作者:Brodbelt, Jennifer S.
Nanohydrophobic Interaction Chromatography Coupled to Ultraviolet Photodissociation Mass Spectrometry for the Analysis of Intact Proteins in Low Charge States
- DOI:10.1021/acs.jproteome.2c00450
- 发表时间:2022-08-31
- 期刊:
- 影响因子:4.4
- 作者:Blevins, Molly S.;Juetten, Kyle J.;Brodbelt, Jennifer S.
- 通讯作者:Brodbelt, Jennifer S.
MS-TAFI: A Tool for the Analysis of Fragment Ions Generated from Intact Proteins
- DOI:10.1021/acs.jproteome.2c00594
- 发表时间:2022-12-14
- 期刊:
- 影响因子:4.4
- 作者:Juetten,Kyle J.;Brodbelt,Jennifer S.
- 通讯作者:Brodbelt,Jennifer S.
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Jennifer Brodbelt其他文献
Jennifer Brodbelt的其他文献
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{{ truncateString('Jennifer Brodbelt', 18)}}的其他基金
REU Site: Frontiers of Materials Chemistry
REU 网站:材料化学前沿
- 批准号:
1852015 - 财政年份:2019
- 资助金额:
$ 63万 - 项目类别:
Standard Grant
REU Site: Frontiers in the Chemistry of Materials
REU 网站:材料化学前沿
- 批准号:
1559839 - 财政年份:2016
- 资助金额:
$ 63万 - 项目类别:
Standard Grant
Ultraviolet Photodissociation Mass Spectrometry
紫外光解离质谱法
- 批准号:
1402753 - 财政年份:2014
- 资助金额:
$ 63万 - 项目类别:
Standard Grant
Photodissociation and Ion Trap Mass Spectrometry
光解离和离子阱质谱分析
- 批准号:
1012622 - 财政年份:2010
- 资助金额:
$ 63万 - 项目类别:
Standard Grant
REU Site: Innovative Material Properties and Chemoselective Targets (IMPACT)
REU 网站:创新材料特性和化学选择性目标 (IMPACT)
- 批准号:
1003947 - 财政年份:2010
- 资助金额:
$ 63万 - 项目类别:
Continuing Grant
IDBR: Development of a Linear Ion Trap/Photodissociation Mass Spectrometer for Biological Research
IDBR:开发用于生物研究的线性离子阱/光解离质谱仪
- 批准号:
0754404 - 财政年份:2008
- 资助金额:
$ 63万 - 项目类别:
Continuing Grant
Photodissociation for Ion Trap Mass Spectrometry
离子阱质谱的光解离
- 批准号:
0718320 - 财政年份:2007
- 资助金额:
$ 63万 - 项目类别:
Continuing Grant
REU Site: Interdisciplinary Research in Cellular and Molecular Imaging
REU 网站:细胞和分子成像的跨学科研究
- 批准号:
0552422 - 财政年份:2006
- 资助金额:
$ 63万 - 项目类别:
Continuing Grant
REU: Interdisciplinary Undergraduate Research in Environmental Science
REU:环境科学跨学科本科研究
- 批准号:
0243659 - 财政年份:2003
- 资助金额:
$ 63万 - 项目类别:
Continuing Grant
Photodissociation in a Quadrupole Ion Trap
四极离子阱中的光解离
- 批准号:
0315337 - 财政年份:2003
- 资助金额:
$ 63万 - 项目类别:
Continuing Grant
相似海外基金
Implementing Photodissociation Mass Spectrometry to study Disease and Lipid Structure
利用光解离质谱研究疾病和脂质结构
- 批准号:
10377907 - 财政年份:2021
- 资助金额:
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Ultraviolet Photodissociation Mass Spectrometry for Characterization of Biological Molecules
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10389836 - 财政年份:2021
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- 批准号:
10320024 - 财政年份:2021
- 资助金额:
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Advancing Ultraviolet Photodissociation Mass Spectrometry for Precision Mapping of Protein Glycosylation
推进紫外光解离质谱技术以精确绘制蛋白质糖基化图谱
- 批准号:
10350600 - 财政年份:2021
- 资助金额:
$ 63万 - 项目类别:
Ultraviolet Photodissociation Mass Spectrometry for Characterization of Biological Molecules
用于表征生物分子的紫外光解离质谱法
- 批准号:
10797256 - 财政年份:2021
- 资助金额:
$ 63万 - 项目类别:
Ultraviolet Photodissociation Mass Spectrometry for Characterization of Biological Molecules
用于表征生物分子的紫外光解离质谱法
- 批准号:
10543449 - 财政年份:2021
- 资助金额:
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Photodissociation mass spectrometry for lipidome analysis
用于脂质组分析的光解离质谱法
- 批准号:
DP190102464 - 财政年份:2019
- 资助金额:
$ 63万 - 项目类别:
Discovery Projects
Ultraviolet Photodissociation Mass Spectrometry
紫外光解离质谱法
- 批准号:
1402753 - 财政年份:2014
- 资助金额:
$ 63万 - 项目类别:
Standard Grant
Photodissociation and Ion Trap Mass Spectrometry
光解离和离子阱质谱分析
- 批准号:
1012622 - 财政年份:2010
- 资助金额:
$ 63万 - 项目类别:
Standard Grant
IDBR: Development of a Linear Ion Trap/Photodissociation Mass Spectrometer for Biological Research
IDBR:开发用于生物研究的线性离子阱/光解离质谱仪
- 批准号:
0754404 - 财政年份:2008
- 资助金额:
$ 63万 - 项目类别:
Continuing Grant