Fluorescence Spectroscopy in the Biochemistry Laboratory

阿尔马学院生物化学系的主要目标是 为本科生提供设备完善的教学设施 生物化学与分子生物学现代技术 遗传学 这个项目的目标是引入 荧光光谱学进入本科生生物化学 实验室作为一个强大的分析工具。请求的 仪器提供快速和灵敏的检测 分子事件允许调查领域的 生物化学，补充目前的方法和 实验室中可用的仪器以及 加强课程的讲授部分， 部门 高级生物化学导论 生物化学和分子遗传学课程， 生物化学系将使用荧光 分光光度计及配套设备。 我们将研究 蛋白质-配体相互作用，酶动力学和蛋白质 变性动力学;为了研究脂质体的性质， 活细胞和生物膜;定量小 核酸量;并探测蛋白质-核酸 交互.除了它在课堂上的作用 经验，荧光分光光度计也将 提高学生的研究机会，阿尔马学院 提供给学生。仪器将显著 加强生物化学实验室的经验，并将提供 学生与一个强大的技术，是未充分利用的， 生物化学实验室目前。

A major goal of the Alma College Biochemistry Department is to provide well equipped facilities to teach undergraduate students modem techniques in biochemistry and molecular genetics. The goal of this project is to introduce fluorescence spectroscopy into the undergraduate biochemistry laboratory as a powerful analytical tool. The requested instrumentation provides rapid and sensitive detection of molecular events allowing investigation into areas of biochemistry that complement current methodology and instrumentation available in the laboratory as well as reinforce the lecture portions of the courses offered in the department. The introductory biochemistry, advanced biochemistry, and molecular genetics courses offered by the biochemistry department will use the fluorescence spectrophotometer and supporting equipment. We will study protein-ligand interactions, enzyme kinetics, and protein denaturation kinetics; to investigate properties of liposomes, living cells, and biological membranes; to quantitate small amounts of nucleic acids; and to probe protein-nucleic acid interactions. In addition to its role in the classroom experience, the fluorescence spectrophotometer will also enhance student research opportunities that Alma College provides its students. The instrumentation will significantly enhance the biochemistry laboratory experience and will provide students with a powerful technique that is underutilized in the undergraduate biochemistry laboratory presently.