Improvement of the Biochemistry Curriculum by Incorporation of Fluoresence and CD Spectroscopy

结合荧光和圆二色光谱改进生物化学课程

• 批准号: 9751530
• 负责人: John Hansen
• 金额: $ 6.38万
• 依托单位: University of West Georgia
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-07-01 至 1999-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9751530&HistoricalAwards=false
• 关键词: Improvement; Biochemistry; Curriculum; Incorporation; Fluoresence

Them Department of Chemistry at the State University of West Georgia is actively pursuing the modernization of its teaching laboratories by providing hands-on experience with modern instrumentation. Presently, we are making major changes in our curriculum as we convert to a semester based system. This provides an ideal opportunity to make dramatic changes in our biochemistry curriculum that address the significant changes that the field has recently undergone. Biochemistry has been revolutionized by techniques developed for manipulating DNA. These techniques have opened avenues of inquiry into the physical chemical nature of biological systems. Thus, physical biochemistry has increasingly become the fundamental basis of biochemical understanding. Since this background is often absent in the education of undergraduate biochemistry majors, we propose to restructure the present curriculum to reflect the contemporary biochemical outlook. In a novel approach we plan to combine the traditional topics of biochemistry with topics in physical biochemistry and spectroscopy of biological macromolecules. In the laboratory course we will use CD and fluorescence spectroscopy to explore areas such as structure, stability and dynamics of macromolecules, enzyme kinetics, protein folding and membrane dynamics. These spectroscopic techniques are not inordinately difficult techniques for students to Learn, and they can be readily applied in a laboratory course. The goal is for our students to be conversant in, and comfortable with a more physical chemical approach to biochemistry. Those that enter graduate school will have the confidence to pursue broader research interests, while those that seek positions in biotechnology will have experience in the instrumental techniques used by the industry. Many of the companies have broadly defined concerns in physical biochemistry (e.g., designing and engineering new enzymes, and minimizing aggregation in the recombinant production of therapeutic proteins).

西格鲁吉亚州立大学化学系通过提供现代仪器的实践经验，积极推进教学实验室的现代化。 目前，我们正在我们的课程，因为我们转换到一个学期为基础的系统的重大变化。 这提供了一个理想的机会，使我们的生物化学课程，解决该领域最近发生的重大变化的戏剧性变化。 生物化学因操纵DNA的技术而发生了革命性的变化。 这些技术开辟了研究生物系统物理化学本质的途径。 因此，物理生物化学已日益成为生物化学认识的根本基础。 由于这一背景往往是缺乏在本科生物化学专业的教育，我们建议重组目前的课程，以反映当代生物化学的前景。 在一个新的方法，我们计划结合联合收割机的生物化学与物理生物化学和生物大分子光谱学的主题的传统主题。 在实验室课程中，我们将使用CD和荧光光谱来探索大分子的结构，稳定性和动力学，酶动力学，蛋白质折叠和膜动力学等领域。 这些光谱技术对学生来说并不是非常难学的技术，而且它们可以很容易地应用于实验室课程。 我们的目标是让我们的学生熟悉，并与更多的物理化学方法生物化学舒适。 那些进入研究生院的人将有信心追求更广泛的研究兴趣，而那些寻求生物技术职位的人将拥有该行业使用的仪器技术的经验。 许多公司在物理生物化学方面有广泛的定义（例如，设计和改造新酶，以及使治疗性蛋白质的重组生产中的聚集最小化）。