Quantifying Adsorption of Peptides at Gas-Liquid Interfaces

快速原子轰击（FAB）质谱法将被评价为一种测量多肽气体-甘油界面表面过量浓度的方法。使用FAB质谱测量的表面浓度将与使用放射性示踪技术测量的表面浓度进行比较，放射性示踪技术是目前唯一用于直接定量气液相蛋白质和肽浓度的方法。如果成功，将获得描述肽在n2 -甘油和真空-甘油界面行为的第一个高度精确的数据。由于非水溶剂中酶合成的化学和生物技术研究和应用，多肽和蛋白质在非水溶剂中的行为越来越重要。除了开发一种新方法外，本研究还将增加对FAB电离基本机制的了解。这项研究将由一名化学家和两名化学工程师与一名蛋白质生物化学家协商后进行。

Fast atom bombardment (FAB) mass spectrometry will be evaluated as a method to measure surface area excess concentration at the gas- glycerol interface of peptides. Surface concentrations measured using FAB MS will be compared to those measured using the radiotracer technique, the only method currently in use to directly quantify the concentration of proteins and peptides at gas-liquid phase. If successful, the first highly accurate data will be acquired describing the behavior of peptides at N2-glycerol and vacuum-glycerol interfaces. The behavior of peptides and proteins in non-aqueous solvents is of increasing importance because of the chemical and biotechnology studies and application of enzymatic synthesis in non-aqueous solvents. In addition to the development of a new method, this research will add knowledge about the fundamental mechanism of FAB ionization. This research will be carried out by a chemist and two chemical engineers in consultation with a protein biochemist.