Quantifying Adsorption of Peptides at Gas-Liquid Interfaces

定量肽在气-液界面的吸附

• 批准号: 9204042
• 负责人: Richard Van Breemen
• 金额: $ 21万
• 依托单位: North Carolina State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-12-15 至 1996-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9204042&HistoricalAwards=false
• 关键词: Quantifying; Adsorption; Peptides; Gas; Liquid

Fast atom bombardment (FAB) mass spectrometry will be evaluated as a method to measure surface area excess concentration at the gas- glycerol interface of peptides. Surface concentrations measured using FAB MS will be compared to those measured using the radiotracer technique, the only method currently in use to directly quantify the concentration of proteins and peptides at gas-liquid phase. If successful, the first highly accurate data will be acquired describing the behavior of peptides at N2-glycerol and vacuum-glycerol interfaces. The behavior of peptides and proteins in non-aqueous solvents is of increasing importance because of the chemical and biotechnology studies and application of enzymatic synthesis in non-aqueous solvents. In addition to the development of a new method, this research will add knowledge about the fundamental mechanism of FAB ionization. This research will be carried out by a chemist and two chemical engineers in consultation with a protein biochemist.

快原子轰击（FAB）质谱法将评价为 一种测量气体表面过量浓度的方法， 肽的甘油界面。 测量的表面浓度 将使用FAB MS测量的结果与使用 放射性示踪剂技术，目前使用的唯一方法， 定量蛋白质和肽在气液 相位 如果成功，第一个高度准确的数据将是 获得描述肽在N2-甘油和 真空-甘油界面。 肽和蛋白质的行为 在非水溶剂中的应用越来越重要，因为 化学和生物技术研究及酶的应用 在非水溶剂中合成。 除了发展 一种新的方法，这项研究将增加知识， FAB电离的基本机制。 这项研究将是 由一名化学家和两名化学工程师协商完成 一个蛋白质生物化学家