Molecular Genetics of Arabidopsis Thaliana

这项提案有四个独立的实验部分，它们的目标都是试图解决问题的不同方面，即一组调控基因活动如何将未分化的花原基转变为许多分化细胞类型的复杂而精确的阵列。拟进行的实验有：1)对我们已克隆的两个花卉调控基因(APETALA3和Pistillata)进行了详细的分子研究；2)克隆和类似地研究地籍基因超人3)寻找对花发育有调控作用的新基因，以及其他与调控基因相互作用的基因，这些基因的研究将揭示与迄今已知的同源异型基因和地籍基因所揭示的一样多的花型形成；以及4)将野生型和突变型花发育的描述带到一个新的细胞分析水平，使用激光扫描共聚焦显微镜，作为回答迄今已完成的分析留下的许多问题的一种方式。%的植物开花然后产生，因此，尽管拟南芥是一种杂草，没有商业意义，但如果这项工作有助于理解花的发育是如何受到基因控制的，其经济意义是重大的。

This proposal has four separate experimental parts, all related by their goal of attempting to solve different aspects of the problem of how a set of regulatory gene activities turns undifferentiated floral primordia into complex and precise arrays of many differentiated cell types. The experiments proposed are 1) to study in great molecular detail two floral regulatory genes that we have already cloned (APETALA3 and PISTILLATA); 2) to clone and similarly study the cadastral gene SUPERMAN 3) to find new genes with regulatory effects on flower development, and others which interact with the regulatory genes, whose study should reveal as much about floral pattern formation as has been revealed by the study of the homeotic and cadastral genes so far known; and 4) to take the description of wild-type and mutant flower development to a new level of cellular analysis, using laser scanning confocal microsopy, as a way of answering many of the questions left unanswered by the analyses that have been done to date. %%% Plants flower and then produce, thus although Arabidopsis is a weed and of no commercial concern, if an understanding of how flower development is genetically controlled results from this work, the economic implications are significant.