Molecular Genetics of Arabidopsis

0211670Meyerowitz提出了一个主要的研究计划，其目标是详细了解植物茎尖分生组织如何控制其细胞分裂的模式和数量。 提出了两组遗传实验，每组都有自己的目标。 第一组包括仔细分析突变体和野生型芽顶端分生组织（SAMs）中的细胞类型和细胞分裂，以及由控制分生组织行为的基因表达变化引起的SAMs中的动态变化的时程分析。 这些基因包括CLAVATA1、CLAVATA3、WUSCHEL和SHOOT MERISTEMLESS。 这些实验应导致在一个新的详细视图细胞分裂和细胞分化控制分生组织，并将提供一个工具包，详细分析新的突变体表型和新的基因功能。 第二组实验是对新发现的分生组织控制基因（如HANABA TARANU和KANADI 4）进行发育遗传分析，并对迄今为止尚未用于植物的类型进行诱变筛选，以找到更多的分生组织控制基因。 这些实验将填补分生组织控制中一些缺失的参与者，并将直接导致分生组织中细胞通讯和行为的新假设。 总体结果预计将是一个新的详细和动态的描述分生组织细胞的活动，并从这一点，并从控制分生组织行为的基因的遗传分析，茎尖分生组织的新功能的理解，从而植物的生长和发育。

0211670MeyerowitzA major research program is proposed, with the goal of understanding in detail how shoot apical meristems of plants control their patterns and numbers of cell divisions. Two sets of genetic experiments are proposed, each with its own aims. The first set includes careful analyses of cell type and cell divisions in mutant and wild type shoot apical meristems (SAMs), and time course analysis of the dynamic changes induced in SAMs by changes in expression of the genes that control meristem behavior. Among these genes are CLAVATA1, CLAVATA3, WUSCHEL, and SHOOT MERISTEMLESS. These experiments should result in a newly detailed view of cell division and cell differentiation control in meristems, and will provide a toolkit for detailed analysis of new mutant phenotypes and new gene functions. The second set of experiments are developmental-genetic analyses of newly discovered meristem control genes such as HANABA TARANU and KANADI4, and mutagenic screens of types not used so far with plants, to find more meristem control genes. These experiments will fill in some of the missing players in meristem control, and will lead directly to new hypotheses of cellular communication and behavior in meristems. The overall result is expected to be a newly detailed and dynamic description of meristem cell activity, and following from this, and from genetic analysis of the genes that control meristem behavior, a new functional understanding of shoot apical meristems, and thus of plant growth and development.