Molecular Genetics of Arabidopsis

拟南芥分子遗传学

• 批准号: 0211670
• 负责人: Elliot Meyerowitz
• 金额: $ 80万
• 依托单位: California Institute of Technology
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-15 至 2005-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0211670&HistoricalAwards=false
• 关键词: Molecular; Genetics; Arabidopsis

0211670MeyerowitzA major research program is proposed, with the goal of understanding in detail how shoot apical meristems of plants control their patterns and numbers of cell divisions. Two sets of genetic experiments are proposed, each with its own aims. The first set includes careful analyses of cell type and cell divisions in mutant and wild type shoot apical meristems (SAMs), and time course analysis of the dynamic changes induced in SAMs by changes in expression of the genes that control meristem behavior. Among these genes are CLAVATA1, CLAVATA3, WUSCHEL, and SHOOT MERISTEMLESS. These experiments should result in a newly detailed view of cell division and cell differentiation control in meristems, and will provide a toolkit for detailed analysis of new mutant phenotypes and new gene functions. The second set of experiments are developmental-genetic analyses of newly discovered meristem control genes such as HANABA TARANU and KANADI4, and mutagenic screens of types not used so far with plants, to find more meristem control genes. These experiments will fill in some of the missing players in meristem control, and will lead directly to new hypotheses of cellular communication and behavior in meristems. The overall result is expected to be a newly detailed and dynamic description of meristem cell activity, and following from this, and from genetic analysis of the genes that control meristem behavior, a new functional understanding of shoot apical meristems, and thus of plant growth and development.

Meyerowitz提出了一个主要的研究计划，目的是详细了解植物顶端分生组织如何控制它们的细胞分裂模式和数量。两套基因实验被提出，每一套都有自己的目标。第一套包括对突变型和野生型顶端分生组织中细胞类型和细胞分裂的仔细分析，以及对控制分生组织行为的基因表达变化所诱导的顶端分生组织中的动态变化的时程分析。这些基因包括CLAVATA1、CLAVATA3、WUSCHEL和地上部MERISTEMLESS。这些实验将导致对分生组织中细胞分裂和细胞分化控制的新的详细的观察，并将为详细分析新的突变表型和新的基因功能提供一个工具箱。第二组实验是对新发现的分生组织控制基因进行发育遗传学分析，如HANABA Taranu和KANADI4，以及迄今尚未用于植物的突变筛选，以寻找更多的分生组织控制基因。这些实验将填补分生组织控制中一些缺失的角色，并将直接导致关于分生组织中细胞通讯和行为的新假说。预计总体结果将是对分生组织细胞活性的新的详细和动态的描述，并由此以及对控制分生组织行为的基因的遗传分析，对茎顶端分生组织的新的功能理解，从而对植物的生长和发育。