CA2+-Mediated Signal Transduction in Plants

CA2 - 植物介导的信号转导

• 批准号: 9205561
• 负责人: Jeffrey Harper
• 金额: $ 15万
• 依托单位: The Scripps Research Institute
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-08-01 至 1995-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9205561&HistoricalAwards=false
• 关键词: CA2+; Mediated; Signal; Transduction; Plants

A new family of Ca2+-dependent protein kinases, called CDPKs, has recently been discovered. CDPKs are unique because they have a novel structure with a kinase domain joined to a Ca2+-binding domain similar to calmodulin. An important long term goal is to understand the function of CDPKs in Ca2+-mediated signal transduction pathways. The model plant system Arabidopsis has been chosen for this purpose. The foundation of the proposal is to determine how Ca2+ regulates CDPK activity. A biochemical and molecular genetic approach will be used to test a model referred to as a "stream-lined version of CaMK II". An E. coli. expression system has been developed to provide functional enzyme for this purpose. Two approaches will be pursued to investigate the biological functions of a specific CDPK isoform: 1) Isoform AK1 and a mutant "unregulated" version of AK1, will be over-expressed in transgenic plants to examine the effects of altering the balance of its activity in vivo. 2) AK1's intracellular location will be determined to provide information on its potential in vivo substrates.

最近发现了一个新的Ca2+依赖性蛋白激酶家族，称为CDPKs。CDPKs是独一无二的，因为它们具有一种新颖的结构，激酶结构域连接到类似于钙调蛋白的Ca2+结合结构域。一个重要的长期目标是了解CDPKs在Ca2+介导的信号转导途径中的功能。为此，我们选择了模式植物系统拟南芥。该建议的基础是确定Ca2+如何调节CDPK活性。生物化学和分子遗传学方法将被用于测试一种被称为“CaMK II流线型版本”的模型。大肠杆菌。为实现这一目的，已经开发了表达系统来提供功能酶。我们将采用两种方法来研究特定CDPK异构体的生物学功能：1)AK1异构体和AK1的突变体“不受管制”版本将在转基因植物中过度表达，以检查改变其体内活性平衡的影响。2) AK1在细胞内的位置将被确定，以提供其在体内潜在底物的信息。