NSF-IOS-BSF: Collaborative Research US/Israel: Transcriptome and post-translational regulation of heat stress tolerance in pollen

NSF-IOS-BSF：美国/以色列合作研究：花粉热应激耐受性的转录组和翻译后调控

基本信息

批准号：
1656774
负责人：
Jeffrey Harper
金额：
$ 77.5万
依托单位：
Board of Regents, NSHE, obo University of Nevada, Reno
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-05-01 至 2023-04-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1656774&HistoricalAwards=false
关键词：
NSF IOS BSF Collaborative Research

项目摘要

The proposed research address a critical world-wide food security challenge by generating fundamental scientific insights of importance to developing crop plants with improved heat-stress tolerance during pollination. By the year 2050, projections indicate that world-wide food production must increase by 70% in order to feed an expected world population of 9 billion people. Temperature stress is a major contributor to crop loss around the world, with pollen infertility being one of the most important underlying causes. Fertilization during plant reproduction is highly sensitive to hot and cold temperatures, with even a single hot day or cold night carrying the potential to disrupt reproductive success. Understanding this vulnerability is significant because most of the world's food supply is derived from seed crops that depend on pollination. The transformative potential of the proposed research is derived from pioneering the use of pollen as a model system to gain molecular and genetic insights into thermo-tolerance mechanisms in plants. Additional contributions to broader impacts will occur through co-mentoring graduate students in a collaboration involving two institutions, the University of Nevada (Reno) and Bar-Ilan University, Israel. Both institutions share a common goal of increasing agricultural productivity in arid land environments.The long-term goal is to understand how different plant cells cope with abiotic stress, and to use that knowledge to improve crop productivity. The focus here is on how pollen sense and respond to heat stress during a limited period of time in which they must grow, locate ovules, and discharge male gametophytes to fertilize egg cells. The central hypothesis guiding the proposed research is that a protective heat-stress response in pollen involves signaling pathways that utilize calcium and ROS (reactive oxygen species) to trigger unique pollen-specific changes in the transcriptome. Specific Aim 1 employs a pollen transmission assay to genetically test more than 80 candidate genes for their potential to increase or decrease pollen fertility during a heat stress. Aim 2 is to identify heat-stress dependent changes in the transcriptome that are correlated to managing or responding to changes in cellular levels of ROS. This aim will involve using a fluorescence-activated cell sorter to isolate sub-populations of heat-stressed pollen that show either high or low levels of ROS, and thereby enable a transcriptome comparison to be made between two subpopulations of cells showing differences in their response to heat. Aim 3 is to use a quantitative proteomics strategy to identify heat-stress dependent changes in post-translational modifications, such as changes in phosphorylation of transcription factors. A unifying goal is to identify candidate genes or post-translational modifications that can be manipulated to improve heat-stress tolerance in pollen.

拟议的研究通过为开发在授粉期间具有提高的热压力耐受性的作物植物的基本科学见解来解决全球范围内的关键粮食安全挑战。到2050年，预测表明，为了养活预期的90亿人口，全球粮食生产必须增加70％。温度压力是全球农作物损失的主要因素，花粉不育症是最重要的根本原因之一。植物繁殖期间的受精对炎热和寒冷的温度高度敏感，即使是一个炎热的白天或寒冷的夜晚，也具有破坏生殖成功的潜力。了解这种脆弱性是重要的，因为世界上大多数粮食供应源于依赖授粉的种子作物。拟议的研究的变革潜力来自于将花粉作为模型系统的使用，以获得对植物中热耐受性机制的分子和遗传见解。通过在内华达大学（内华达大学）和以色列巴尔 - 伊兰大学（University）的两家机构中，将通过同事的合作，对更广泛影响的其他贡献。这两个机构都有提高农业生产力在干旱土地环境中的共同目标。长期目标是了解不同的植物细胞如何应对非生物压力，并利用这些知识来提高作物生产率。这里的重点是在必须生长，定位胚珠和排放雄性配子体以使卵细胞施肥的有限时间内，花粉感和对热应激的反应如何。指导拟议的研究的中心假设是，花粉中的保护性热应力反应涉及利用钙和ROS（活性氧）触发转录组中独特的花粉特异性变化的信号通路。特定的目标1采用花粉传递测定法，以基因测试80多个候选基因，以增加或降低热应激期间花粉生育能力的潜力。目的2是确定转录组中与热应力的变化，这些变化与管理或响应ROS的细胞水平变化相关。该目的将涉及使用荧光激活的细胞分选蛋白来分离热压力花粉的亚群，该子花粉显示出很高或低水平的ROS，从而使转录组比较在两个细胞的两个亚群之间进行比较，显示出对热反应的差异。目的3是使用定量蛋白质组学策略来识别翻译后修饰的依赖性压力变化，例如转录因子的磷酸化变化。一个统一的目标是确定可以操纵的候选基因或翻译后修饰，以提高花粉中的热压力耐受性。

项目成果

期刊论文数量（16）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Decapitation Crosses to Test Pollen Fertility Mutations for Defects in Stigma-style Penetration

断头杂交测试花粉育性突变的柱头式渗透缺陷

DOI：
10.1007/978-1-0716-0672-8_3
发表时间：
2020
期刊：
Methods and protocols
影响因子：
2.4
作者：
Weigand, Chyrstle;Harper, Jeffrey F
通讯作者：
Harper, Jeffrey F

A comparison of heat-stress transcriptome changes between wild-type Arabidopsis pollen and a heat-sensitive mutant harboring a knockout of cyclic nucleotide-gated cation channel 16 (cngc16).

DOI：
10.1186/s12864-018-4930-4
发表时间：
2018-07-24
期刊：
BMC genomics
影响因子：
4.4
作者：
Rahmati Ishka M;Brown E;Weigand C;Tillett RL;Schlauch KA;Miller G;Harper JF
通讯作者：
Harper JF

Investigation of the biochemical controls on mercury uptake and mobility in trees

树木汞吸收和迁移的生化控制研究

DOI：
10.1016/j.scitotenv.2022.158101
发表时间：
2022
期刊：
Science of The Total Environment
影响因子：
9.8
作者：
Gustin, Mae Sexauer;Dunham-Cheatham, Sarrah M.;Harper, Jeffrey F.;Choi, Won-Gyu;Blum, Joel D.;Johnson, Marcus W.
通讯作者：
Johnson, Marcus W.

Direct analysis of pollen fitness by flow cytometry: implications for pollen response to stress