Acquisition of a Circular Dichroism Spectrometer

要求为微处理器控制、可变温度、记录圆二色谱的光谱仪提供部分资金。该仪器将由首席调查员(Michael Y.Ogawa)每天监督，他将使用它来确定为电子转移研究衍生的合成多肽的构象行为。该仪器还将被联合首席研究员(N.B.Leontis)广泛用于监测新型核酸结构的构象行为，如三向DNA和RNA连接及其与插层配体的相互作用。此外，BGSU生物科学系的George Bullerjahn将使用该仪器来监测光合膜的结构转变。还描述了来自北京国立大学化学系和托莱多大学化学系的其他用户的小项目。在俄亥俄州西北部没有类似的仪器。

Partial funding for a microprocessor-controlled, variable- temperature, recording circular dichroism spectrometer is requested. The instrument will be supervised on a daily basis by the Principal Investigator (Michael Y. Ogawa) who will use it to determine the conformational behavior of synthetic polypepties derivatized for electron-transfer studies. The instrument will also be used extensively by the co-Principal Investigator (N. B. Leontis) to monitor the conformational behavior of novel nucleic acid structures such as three-way DNA and RNA junctions and their interactions with intercalating ligands. In addition, the instrument will be used by George Bullerjahn in the Department of Biological Sciences at BGSU to monitor structural transitions in photosynthetic membranes. Minor projects from other users in the Department of Chemistry at both BGSU and the University of Toledo are also described. There is no comparable instrumentation available in Northwest Ohio.