Electron-Transfer Across Artificial Beta-Sheets

人工β片上的电子转移

• 批准号: 9307791
• 负责人: Michael Ogawa
• 金额: $ 21万
• 依托单位: Bowling Green State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-07-15 至 1997-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9307791&HistoricalAwards=false
• 关键词: Electron; Transfer; Across; Artificial; Beta

9307791 Ogawa Professor Ogawa of Bowling Green State University will investigate electron transfer across artificial beta-pleated sheets with support from the Inorganic, Bioinorganic, and Organometallic Chemistry Program. He will synthesize compounds based on oligopeptides; these will be joined in pairs at the ends so as to induce the beta-pleated sheet conformation. Electron transfer donor and acceptor sites will be attached to these capped oligopeptides so that the role of beta pleated sheets in mediating electron transfer can be assessed. The molecules will be characterized by circular dichroism and multinuclear nmr spectroscopy, and where possible by x-ray diffraction. Electron transfer will be initiated by flash photolysis and monitored by fluorescence and absorption spectroscopy. The primary goal will be to determine if beta-pleated sheets are unusually effective in mediating electron transfer, as has been predicted theoretically. %%% The transfer of an electron through proteins is an essential component of many life processes. Often this must occur through distances that are long on the atomic scale. It has been suggested that certain structural motifs within proteins are especially effective in fostering such long range electron transfer events. In particular, structures known as "beta-pleated sheets" have been implicated. Experiments to test this hypothesis will be conducted by designing and synthesizing artificial beta-pleated sheets; these molecules will be constructed with sites that are able to participate in electron transfer reactions. The rates of electron transfer will then be measured and compared with those of similar molecules that lack the beta-pleated sheets. The information to be obtained will refine our understanding of the ways that proteins perform their functions. ***

9307791小川教授保龄球绿色州立大学小川将研究电子转移跨越人工β-折叠片的支持下，无机，生物无机和有机化学计划。 他将合成基于寡肽的化合物;这些化合物将在末端成对连接，以诱导β折叠构象。 电子转移供体和受体位点将连接到这些加帽的寡肽，以便可以评估β折叠片在介导电子转移中的作用。 分子将通过圆二色性和多核核磁共振光谱来表征，并且在可能的情况下通过x射线衍射来表征。 电子转移将通过闪光光解引发，并通过荧光和吸收光谱法进行监测。 主要目标是确定β-折叠片是否如理论上预测的那样在介导电子转移方面异常有效。 电子通过蛋白质的转移是许多生命过程的重要组成部分。 通常，这必须通过原子尺度上的长距离来实现。 有人认为，蛋白质内的某些结构基序在促进这种长距离电子转移事件方面特别有效。 特别是，被称为“β-褶皱片”的结构已经被牵涉。 测试这一假设的实验将通过设计和合成人工β-折叠片来进行;这些分子将被构建为具有能够参与电子转移反应的位点。 然后测量电子转移的速率，并与缺乏β折叠片的类似分子进行比较。 所获得的信息将使我们更好地理解蛋白质发挥功能的方式。 ***