Fluorescence Microscopic Visualization of Lipid Domains

这个建议是为了确定酰基的相对重要性 链和磷脂头部基团在脂质的形成 红细胞膜和模型膜中的结构域。荧光 数字成像显微镜和稳态荧光偏振 将使用zation技术。荧光标记磷脂 其头基和酰基链不同， 购买，和分布对不同的任何头部 基团或酰基链将通过这两种技术测定。三 结果是可能的，并应允许解决不同的 这两种技术的结果。 %%% 所有的细胞膜都含有一种叫做磷脂的化合物， 其具有两种不同的组分：偏好水的基团， 因为它们有带电的部分， 脂肪.目前尚不清楚这两个部分是否同样重要- 或者一个很重要，另一个不那么重要。 实验计划使用两种完全不同的技术。两 测量特别标记的磷脂的环境。 这些脂质的混合物将与模型膜一起使用， 从红细胞中分离膜，看看这两种成分 是否对膜中的定位有同等的贡献。

This proposal is to determine the relative importance of acyl chains and phospholipid head groups in the formation of lipid domains in erythrocyte membranes and model membranes. Fluorescence digital imaging microscopy and steady state fluorescence polari- zation techniques will be used. Fluorescently labeled phospholipids which differ in head groups and acyl chains will be synthesized or purchased, and the distribution of pairs differing in either head groups or acyl chains will be determined by both techniques. Three results are possible, and should allow resolution of disparate results obtained by the two techniques. %%% All cellular membranes contain compounds known as phospholipids, which have two distinct components: groups which prefer water, because they have charged parts, and groups which are components of fats. It is not clear whether the two components are equally impor- tant, or whether one is very important and the other is less so. Experiments are planned using two quite different techniques. Both measure the environment of specially labeled phospholipids. Mixtures of these lipids will be used with model membranes and membranes from red blood cells, to see if both components contribute equally to positioning in the membranes or not.