Molecular Biology and Physiology of Neuronal Modulation

神经元调节的分子生物学和生理学

• 批准号: 9209785
• 负责人: Yasuko Nakajima
• 金额: $ 20.09万
• 依托单位: University of Illinois at Chicago
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-08-15 至 1996-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9209785&HistoricalAwards=false
• 关键词: Molecular; Biology; Physiology; Neuronal; Modulation

There are many transmitter substances which regulate the activity of nerve cells of the central nervous system. The objective of this proposal is to elucidate the mechanisms by which these transmitter substances activate intracellular messenger processes that lead to the modulation of excitability through their action on potassium channel conductance properties. Recent studies have shown that excitability of neurons is dependent on potassium channels which are, in turn, regulated by G-proteins called Gi and Go, of which there are several subtypes. The specific aim of this investigation is to identify which of the G-protein subtypes are related to neurotransmitter action (acetylcholine and somatostatin). Molecular biological techniques will be used to produce specific mutations to selectively inactivate G-proteins and thereby determine which of them is directly affected by the neurotransmitters to act on potassium conductance of the cell. For this purpose mutated alpha subunits will be inserted into AtT-20 cells, a pituitary tumor cell line which contain G-protein regulated potassium channels. The effect of the mutants can then be ascertained on potassium channel function.

有许多递质物质调节中枢神经系统神经细胞的活动。 该提案的目的是阐明这些递质物质激活细胞内信使过程的机制，从而通过其对钾通道电导特性的作用来调节兴奋性。 最近的研究表明，神经元的兴奋性取决于钾通道，而钾通道又受称为 Gi 和 Go 的 G 蛋白调节，其中有多种亚型。 这项研究的具体目的是确定哪些 G 蛋白亚型与神经递质作用（乙酰胆碱和生长抑素）相关。 分子生物学技术将用于产生特定的突变，选择性地灭活G蛋白，从而确定其中哪些直接受神经递质影响，从而作用于细胞的钾电导。 为此，突变的 α 亚基将被插入 AtT-20 细胞中，AtT-20 细胞是一种含有 G 蛋白调节钾通道的垂体肿瘤细胞系。 然后可以确定突变体对钾通道功能的影响。