Fluorescence spectrometer for advanced research in plant physiology, biochemistry and molecular biology
用于植物生理学、生物化学和分子生物学高级研究的荧光光谱仪
基本信息
- 批准号:405914-2011
- 负责人:
- 金额:$ 2.41万
- 依托单位:
- 依托单位国家:加拿大
- 项目类别:Research Tools and Instruments - Category 1 (<$150,000)
- 财政年份:2010
- 资助国家:加拿大
- 起止时间:2010-01-01 至 2011-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The requested Perkin-Elmer LS-55 Fluorescence Spectrometer will be used in the measurement of enzyme activities, determination of metabolite concentrations in subcellular compartments of plant cells, determination of kinetic parameters of enzymes and calculation of metabolic fluxes in the cell. The instrument will be placed in the Laboratory of Plant Metabolism at the Department of Biology, Memorial University of Newfoundland. The LS-55 Fluorescence Spectrometer is capable of measuring diverse biophysical/biochemical parameters and is therefore extremely versatile. It provides high-resolution readouts with low background noise, which are extremely accurate and reproducible, characteristics essential for state of the art research on plant/microbial metabolism and bioenergetics. It is a powerful tool for the investigation of enzyme kinetics and calculation of metabolic fluxes based on the metabolic control theory. It will allow us to evaluate the intensities of cellular metabolic pathways in stress-induced processes under different conditions. The requested piece of equipment is an integral component of most physiological and biochemical laboratories aimed at understanding the operation of cellular organelles involved in bioenergetics, such as mitochondria and peroxisomes. The availability of the LS-55 Fluorescent Spectrometer will promote research on adaptation of plants and microorganisms to environmental stresses. This research has a major ecological, industrial and evolutionary significance.
所要求的Perkin-Elmer LS-55荧光光谱仪将用于测量酶活性、测定植物细胞亚细胞区室中的代谢物浓度、测定酶的动力学参数和计算细胞中的代谢通量。该仪器将被放置在纽芬兰纪念大学生物系植物代谢实验室。LS-55荧光光谱仪能够测量各种生物物理/生物化学参数,因此用途非常广泛。它提供了高分辨率的低背景噪声读数,这是非常准确和可重复的,对植物/微生物代谢和生物能量学的最新研究的基本特征。它是基于代谢控制理论研究酶动力学和计算代谢通量的有力工具。这将使我们能够评估在不同条件下应激诱导过程中细胞代谢途径的强度。所要求的设备是大多数生理和生化实验室的组成部分,旨在了解生物能量学中涉及的细胞器,如线粒体和过氧化物酶体的运作。LS-55荧光光谱仪的问世将促进植物和微生物对环境胁迫适应性的研究。这项研究具有重大的生态、工业和进化意义。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Igamberdiev, Abir其他文献
Igamberdiev, Abir的其他文献
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{{ truncateString('Igamberdiev, Abir', 18)}}的其他基金
Nitric oxide metabolism and its interaction with reactive oxygen species during the adaptation of plants to abiotic stress
植物适应非生物胁迫过程中一氧化氮代谢及其与活性氧的相互作用
- 批准号:
RGPIN-2021-02945 - 财政年份:2022
- 资助金额:
$ 2.41万 - 项目类别:
Discovery Grants Program - Individual
Nitric oxide metabolism and its interaction with reactive oxygen species during the adaptation of plants to abiotic stress
植物适应非生物胁迫过程中一氧化氮代谢及其与活性氧的相互作用
- 批准号:
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$ 2.41万 - 项目类别:
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Nitric oxide turnover and the strategy of physiological adaptation of plants to low oxygen stress
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355753-2013 - 财政年份:2018
- 资助金额:
$ 2.41万 - 项目类别:
Discovery Grants Program - Individual
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530664-2018 - 财政年份:2018
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$ 2.41万 - 项目类别:
Engage Grants Program
Biochemical Analysis of Birch Sap and Chaga
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- 批准号:
506402-2016 - 财政年份:2016
- 资助金额:
$ 2.41万 - 项目类别:
Engage Grants Program
Nitric oxide turnover and the strategy of physiological adaptation of plants to low oxygen stress
一氧化氮周转与植物低氧胁迫生理适应策略
- 批准号:
355753-2013 - 财政年份:2016
- 资助金额:
$ 2.41万 - 项目类别:
Discovery Grants Program - Individual
Nitric oxide turnover and the strategy of physiological adaptation of plants to low oxygen stress
一氧化氮周转与植物低氧胁迫生理适应策略
- 批准号:
355753-2013 - 财政年份:2015
- 资助金额:
$ 2.41万 - 项目类别:
Discovery Grants Program - Individual
Nitric oxide turnover and the strategy of physiological adaptation of plants to low oxygen stress
一氧化氮周转与植物低氧胁迫生理适应策略
- 批准号:
355753-2013 - 财政年份:2014
- 资助金额:
$ 2.41万 - 项目类别:
Discovery Grants Program - Individual
Nitric oxide turnover and the strategy of physiological adaptation of plants to low oxygen stress
一氧化氮周转与植物低氧胁迫生理适应策略
- 批准号:
355753-2013 - 财政年份:2013
- 资助金额:
$ 2.41万 - 项目类别:
Discovery Grants Program - Individual
Mitochondrial regulation of nitrogen metabolism under hypoxic conditions
缺氧条件下线粒体对氮代谢的调节
- 批准号:
355753-2008 - 财政年份:2012
- 资助金额:
$ 2.41万 - 项目类别:
Discovery Grants Program - Individual
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