SGER: Direct Sequencing of Albumin

SGER：白蛋白直接测序

• 批准号: 9213289
• 负责人: Linda Maxson
• 金额: $ 6万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-04-15 至 1993-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9213289&HistoricalAwards=false
• 关键词: SGER; Direct; Sequencing; Albumin

This study proposes to collect direct sequence information from the coding portion of the gene for vertebrate serum albumin. Initially DNA (genes) from three frogs will be purified and cloned. Once serum albumin DNA sequences are obtained for these three species, the sequences will be aligned so that regions of complete similarity can be identified. If such identical regions can be found, primers will be developed to allow the amplification of homologous albumin genes from other frogs. The development of such primers will permit the rapid amplification and sequencing of this major nuclear gene from a large number of frog species. This information can be invaluable in assessing the relationships of major groups of organisms which are used extensively in studies of genetics and development.

这项研究建议从脊椎动物血清白蛋白基因的编码部分收集直接序列信息。最初，三种青蛙的DNA(基因)将被提纯和克隆。一旦获得了这三个物种的血清白蛋白DNA序列，就会对序列进行比对，以便能够识别出完全相似的区域。如果可以找到这样的相同区域，将开发出允许从其他青蛙中扩增同源白蛋白基因的引物。这种引物的开发将使从大量青蛙物种中快速扩增和测序这一主要核基因成为可能。这些信息在评估在遗传学和发育研究中广泛使用的主要生物群之间的关系方面可能是非常有价值的。