Acquisition of Equipment for Isolation of Recombinant Proteins

购置重组蛋白分离设备

• 批准号: 9216996
• 负责人: Peter Holloway
• 金额: $ 6万
• 依托单位: University of Virginia Main Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-05-01 至 1995-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9216996&HistoricalAwards=false
• 关键词: Acquisition; Equipment; Isolation; Recombinant; Proteins

Advances in molecular cloning technologies have made it possible to generate large quantities of diverse proteins in micro-organisms, in order to study the biological and physical properties of these proteins. The Department of Biochemistry at the University of Virginia has a long history of biophysical studies on diverse biological systems and several of the faculty, particularly the faculty recently recruited, have research projects designed around studies of such recombinant proteins. Of the 13 full time faculty, 8 are either using recombinant proteins or plan to use them in the near future. One problem with this approach to the generation of research material is in assembling the hardware necessary for the isolation of these proteins from bacterial, or other types of, cells. We have not had a long history of bacteriology or large scale protein purification and so our resources which were once adequate for our biochemical needs are now completely inadequate for generation of recombinant proteins. This shortfall is compounded by the fact that for many of the projected biophysical studies particularly large amounts of protein are required. We propose to purchase the basic instrumentation for moderate scale isolation of recombinant proteins from E. coli, yeast, and other cells.

分子克隆技术的进步使其 可以生成 微生物中的大量不同蛋白质， 为了研究生物和物理特性， 这些蛋白质。生物化学系 弗吉尼亚大学有着悠久的生物物理学历史， 对不同生物系统的研究， 教师，特别是最近招聘的教师， 研究项目设计围绕这样的研究 重组蛋白在13名全职教师中，有8名是 无论是使用重组蛋白，还是计划将其用于 近期 这种研究方法的一个问题是， 材料处于 组装隔离这些设备所需的硬件， 来自细菌或其他类型细胞的蛋白质。我们有 没有很长的细菌学历史， 蛋白质纯化和我们的资源， 足以满足我们的生化需求， 不足以产生重组蛋白。这 由于许多人认为， 预计的生物物理研究，特别是大量的 蛋白质是必需的。 我们建议购买基本仪器， 从大肠杆菌中中等规模分离重组蛋白。 大肠杆菌、酵母和其他细胞。