Acquisition of Equipment for Isolation of Recombinant Proteins

分子克隆技术的进步，使得在微生物体内大量生成不同的蛋白质成为可能，从而研究这些蛋白质的生物学和物理性质。弗吉尼亚大学的生物化学系在生物物理研究方面有着悠久的历史，研究的对象是不同的生物系统，一些教员，尤其是新近招募的教员，都有围绕这些重组蛋白的研究项目。在13名全职教师中，有8名正在使用重组蛋白或计划在不久的将来使用它们。这种方法产生研究材料的一个问题是组装从细菌或其他类型的细胞中分离这些蛋白质所必需的硬件。我们没有很长的细菌学或大规模蛋白质纯化的历史，所以我们曾经足以满足我们生化需求的资源现在完全不足以产生重组蛋白质。由于许多计划中的生物物理研究需要特别大量的蛋白质，这一事实使这种不足更加严重。我们建议购买基础仪器，用于从大肠杆菌、酵母和其他细胞中中等规模分离重组蛋白。

Advances in molecular cloning technologies have made it possible to generate large quantities of diverse proteins in micro-organisms, in order to study the biological and physical properties of these proteins. The Department of Biochemistry at the University of Virginia has a long history of biophysical studies on diverse biological systems and several of the faculty, particularly the faculty recently recruited, have research projects designed around studies of such recombinant proteins. Of the 13 full time faculty, 8 are either using recombinant proteins or plan to use them in the near future. One problem with this approach to the generation of research material is in assembling the hardware necessary for the isolation of these proteins from bacterial, or other types of, cells. We have not had a long history of bacteriology or large scale protein purification and so our resources which were once adequate for our biochemical needs are now completely inadequate for generation of recombinant proteins. This shortfall is compounded by the fact that for many of the projected biophysical studies particularly large amounts of protein are required. We propose to purchase the basic instrumentation for moderate scale isolation of recombinant proteins from E. coli, yeast, and other cells.