Acquisition of a Spectrofluorometer

购买荧光分光光度计

• 批准号: 9016177
• 负责人: Peter Holloway
• 金额: $ 3.8万
• 依托单位: University of Virginia Main Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-01-01 至 1992-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9016177&HistoricalAwards=false
• 关键词: Acquisition; Spectrofluorometer

The group of investigators listed in this proposal have developed, over the years, many techniques for examining various aspects of membrane structure and function. Steady state fluorescence measurements are a fundamental measurement which is ideally suited to membranous systems and this application will describe several diverse techniques which have been, and will be, used to investigate a series of membrane problems from the most fundamental lipid-lipid interaction to the most complex of cell signalling processes. These projects include: the formation of compositional lipid domains in multilipid systems, the conformation of membrane-bound proteins, the mechanism of protein-lipid interactions, the role of lipid physical properties in the functioning of an integral membrane protein and the mechanism of intracellular signalling. In many of the projects involving membrane proteins, advantage will be taken of site-specific mutagenesis to produce modified proteins with more favorable fluorescent properties.

多年来，本提案中列出的研究人员已经开发了许多技术来检查膜结构和功能的各个方面。稳态荧光测量是一种非常适合膜系统的基本测量方法，该应用程序将描述几种不同的技术，这些技术已经并将用于研究一系列膜问题，从最基本的脂质相互作用到最复杂的细胞信号传导过程。这些项目包括：多脂质系统中组成脂质结构域的形成，膜结合蛋白的构象，蛋白-脂质相互作用的机制，脂质物理特性在整体膜蛋白功能中的作用以及细胞内信号传导的机制。在许多涉及膜蛋白的项目中，将利用位点特异性诱变的优势来生产具有更有利荧光特性的修饰蛋白。