The Reproductive Ecology of Fucoid Algae: Fertilization and Early Embryogenesis

岩藻类藻类的繁殖生态学：受精和早期胚胎发生

• 批准号: 9216981
• 负责人: Susan Brawley
• 金额: $ 33.2万
• 依托单位: University of Maine
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-05-01 至 1997-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9216981&HistoricalAwards=false
• 关键词: Reproductive; Ecology; Fucoid; Algae; Fertilization

Although most marine organisms have external fertilization, very little is know about the processes that control fertilization success and early embryonic survival. Inter tidal fucoid algae represent an ideal model system in which to study these processes in nature. Periods of gamete release, current velocities during fertilization, and degree of aggregation of male/female plants will be determined and /or varied experimentally to determine how they influence fertilization success, which will be determined cytological. Whether fertilization can adapt to low salinity environments is unknown; of particular interest is how the polyspermy block is affected, because this is Na+-dependent in may marine species. Electrophysiological and physiological techniques will be used to study the polyspermy block in fucoid algae from low salinity habitats. The ability of embryos of intertidal algae to survive the stresses encountered in the intertidal zone is poorly known. Stage- specific survival of fucoid embryos during low tidal emersion will be studied in the laboratory and in nature. The role of the hormone abscisic acid (ABA) in conferring tolerance to desiccation and freezing will be examined by quantitative immunoassay and by treating sensitive stages with exogenous ABA. Taken together with existing data, this work will offer an unusually complete understanding of the ecological processes affecting recruitment of a marine organism.

虽然大多数海洋生物都有外部 受精，很少有人知道的过程， 控制受精成功和早期胚胎存活。 潮间岩藻是一种理想的模式系统， 在自然界中研究这些过程。 配子周期 施肥过程中的释放、流速和程度 将确定雄性/雌性植物的聚集， /或通过实验改变以确定它们如何影响 受精成功，这将由细胞学决定。 施肥能否适应低盐环境 是未知的;特别感兴趣的是多精受精是如何 阻滞受到影响，因为这是Na+依赖性的， 海洋物种。 电生理和生理 技术将被用来研究多精受精块， 低盐度生境的岩藻。 的能力 潮间带藻类的胚胎在压力下存活 在潮间带遇到的情况知之甚少。阶段- 低潮期岩藻胚胎的比存活率 将在实验室和自然界中研究再现。 激素脱落酸（阿坝）在赋予 干燥和冷冻耐受性将通过 定量免疫测定和通过治疗敏感阶段 外源阿坝。 结合现有数据， 工作将提供一个不寻常的完整的理解， 影响海洋生物补充的生态过程 有机体