A Study of Thymic Nurse Cell Function

胸腺护理细胞功能的研究

• 批准号: 9218859
• 负责人: Jerry Guyden
• 金额: --
• 依托单位: CUNY City College
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-07-15 至 1995-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9218859&HistoricalAwards=false
• 关键词: Study; Thymic; Nurse; Cell; Function

9218859 Guyden Thymic nurse cells (TNCs) are specialized epithelial components that contain T cells completely enclosed in intra-cytoplasmic vacuoles. Very little information about their function has been reported because of the lack of pure TNC populations in quantities necessary for their thorough analyses. Recently, this laboratory has been able to immortalize TNCs with SV40 virus. Cells from these TNC lines have been shown to maintain the ability to internalize thymocytes in vitro. Further, it has been shown that the only cells bound by an internalized into TNCs are CD4- positive, CD8-positive thymocytes. These cell lines will be used to study TNC function. The goals of this research are to determine the molecular components involved in TNC binding and internalization of thymocytes, and to determine the specific function of TNC internalization of double positive thymocytes in T cell development. Two strategies will be employed: (1) TNC- specific monoclonal antibodies (mAb) will be generated in an attempt to identify the receptor/ligand involved in binding and internalization. (2) Antibody preparations shown to block binding of thymocytes to TNCs will be used to clone the gene(s) expressing the protein(s) involved. These studies are designed to determine the function of thymocyte binding and internalization by TNCs. %%% Thymic nurse cells are specialized epithelial cells of the thymus in which T-lymphocytes are regularly found completely enclosed in intra-cytoplasmic vacuoles. This is a very unusual phenomenon in physiology. Very little information about the function of thymic nurse cells is known because of the lack of pure TNC populations in quantities necessary for their thorough analyses. However, they are believed to play an important role in the development of T- lymphocytes of the immune system. With prior NSF support, this laboratory has been able to grow pure populations of thymic nurse cells in culture. The goals of this project a re to study the interaction of thymic nurse cells with T-lymphocytes at the molecular level. The results of this research should provide unique new information on how thymic nurse cells function. ***

9218859 Guyden胸腺滋养细胞（TNC）是特化的上皮成分，其包含完全封闭在胞质内空泡中的T细胞。由于缺乏对其进行彻底分析所需的纯跨国公司数量，关于其功能的资料报道甚少。最近，该实验室已经能够用SV 40病毒使跨国公司永生化。来自这些TNC系的细胞已经显示出在体外保持内化胸腺细胞的能力。此外，已经表明，被内化到TNCs中的结合的细胞仅是CD 4阳性、CD 8阳性胸腺细胞。这些细胞系将用于研究TNC功能。本研究的目的是确定参与TNC结合和胸腺细胞内化的分子组分，并确定TNC内化双阳性胸腺细胞在T细胞发育中的特异性功能。将采用两种策略：（1）将产生TNC特异性单克隆抗体（mAb），以试图鉴定参与结合和内化的受体/配体。(2)显示阻断胸腺细胞与TNCs结合的抗体制剂将用于克隆表达所涉及蛋白质的基因。这些研究的目的是确定胸腺细胞的结合和内化功能的跨国公司。胸腺滋养细胞是胸腺的特化上皮细胞，其中T淋巴细胞经常被发现完全封闭在胞质内空泡中。这在生理学上是一个非常不寻常的现象。关于胸腺哺育细胞功能的信息很少，因为缺乏对其进行彻底分析所需的纯TNC群体。然而，它们被认为在免疫系统的T淋巴细胞的发育中起重要作用。在先前NSF的支持下，该实验室已经能够在培养物中培养纯的胸腺养育细胞群。本课题的目的是从分子水平研究胸腺滋养细胞与T淋巴细胞的相互作用。这项研究的结果应该提供独特的新信息，如何胸腺护士细胞的功能。***