Muscle Development in Squid: An Integrated Analysis of Muscle Fiber Specialization

鱿鱼的肌肉发育：肌纤维专门化的综合分析

• 批准号: 9219495
• 负责人: William Kier
• 金额: $ 29.8万
• 依托单位: University of North Carolina at Chapel Hill
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-03-01 至 1997-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9219495&HistoricalAwards=false
• 关键词: Muscle; Development; Squid; Integrated; Analysis

The development and differentiation of two muscle fiber types in the squid Sepioteuthis lessoniana will be studied. First, transmission electron microscopy will be used to document the differentiation of the cross-striated muscle cells of the tentacles and the obliquely striated muscle cells of the arms. Second, the differentiation of these two muscle cell types will be correlated with behavioral changes that occur during development and growth using high speed video recording of prey capture. Third, immunocytochemical techniques will be used to document the pattern and timing of expression of alpha-actinin and paramyosin, proteins important in determining the dimensions and organization of the muscle cell filaments. This integrated approach to muscle fiber differentiation will provide the most comprehensive account of cephalopod muscle development to date. It will afford a unique opportunity to study the transition between two muscle striation types and will examine the significance of the structural characteristics of muscle cells specialized for a particular function. In addition, it will examine the mechanisms controlling the assembly of two distinct muscle cells types during development and will, therefore, provide fundamental insight into the mechanisms of muscle cell growth and development. Because the study will integrate analyses at several levels of organization, the physiological significance of the diversity of structural and biochemical components of muscle can be explored precisely.

本文将研究鱿鱼两种肌肉纤维类型的发育和分化。首先，利用透射电子显微镜记录触须横纹肌细胞和手臂斜纹肌细胞的分化。其次，这两种肌肉细胞类型的分化将与在发育和生长过程中发生的行为变化相关，使用捕获猎物的高速录像。第三，免疫细胞化学技术将用于记录α -肌动蛋白和副肌凝蛋白的表达模式和时间，这两种蛋白在决定肌肉细胞丝的尺寸和组织方面很重要。这种肌纤维分化的综合方法将提供迄今为止头足类动物肌肉发育的最全面的描述。它将提供一个独特的机会来研究两种肌肉条纹类型之间的转变，并将检查为特定功能专门的肌肉细胞结构特征的意义。此外，它将研究在发育过程中控制两种不同肌肉细胞类型组装的机制，从而为肌肉细胞生长和发育的机制提供基本的见解。由于该研究将整合多个组织水平的分析，因此可以精确地探索肌肉结构和生化成分多样性的生理意义。