Introduction of Molecular Biology to the Undergraduate Curriculum

分子生物学本科课程简介

• 批准号: 9251263
• 负责人: James Pierce
• 金额: $ 1.61万
• 依托单位: Texas A&M University-Kingsville
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-06-01 至 1994-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9251263&HistoricalAwards=false
• 关键词: Introduction; Molecular; Biology; Undergraduate; Curriculum

This project supplements a current molecular biology course with a hands-on labortory focusing on experiments that will lead to undergraduate research projects. Equipment includes: horizontal gel electrophoresis (1/student pair, Hoeffer), photographic materials (Polaroid), a microcentrifuge (Eppendorf), a benchtop incubator (Precision) and material for PCR (thermal cycler, vacuum blotter). Exercises include: plasmid isolation, restriction mapping, Southern hybridizations, construction of cDNA libraries, screening techniques and PCR reactions. The institution has a high enrollment of minorities (59% Hispanic).

这个项目是对当前分子生物学课程的补充，它提供了一个专注于实验的动手实验室，这些实验将导致本科生的研究项目。设备包括：水平凝胶电泳（1/学生对，Hoeffer），照相材料（宝丽来），微离心机（Eppendorf），台式培养箱（Precision）和PCR材料（热循环仪，真空吸盘）。练习包括：质粒分离，限制性内切酶定位，Southern杂交，cDNA文库的构建，筛选技术和PCR反应。该机构的少数族裔入学率很高（59%为西班牙裔）。