The Integration of DNA Microarray Technology in the Undergraduate Curriculum

生物科学（61）本项目将DNA微阵列技术和生物信息学数据分析的应用作为USP本科生物学、生物化学和生物信息学课程的核心技术。微阵列技术是研究基因表达和分子诊断的重要生物技术工具，并在制药和生物技术行业进行药物发现和开发的方式上启动了范式转变。为了满足生物科学界当前和未来的需求，一个由微阵列仪器、试剂和生物信息公司组成的完整产业已经发展起来。最近，已经开发了用于制造微阵列（“网格”）和微阵列读取器（“扫描仪”）的仪器，这些仪器在小型实验室环境中销售和定价。21世纪的生命科学专业毕业生需要了解微阵列技术的科学原理和实用技术技能。我们的跨学科课程计划采用微阵列技术，并在分子生物学（BI-290），遗传学（BS-466），生物信息学（BI-450）三门课程中开发微阵列特定练习，并支持我们的定向研究计划（BS-499和CH-450）的本科生研究。这个项目是我们新的生物信息学学士学位的教育基础的一部分，让生物学和生物化学项目的学生有机会在他们的第二，第三或第四年的学习中体验微阵列技术。学生进行基因表达和DNA诊断实验，并使用生物信息学软件来组织和挖掘微阵列数据。这一建议的成功实施将导致DNA微阵列练习扩展到其他课程和项目(如。微生物学)。该项目提供了一个课程模板，为其他本科院校提供协议和数据集。

Biological Sciences (61)This project integrates DNA microarray technology and the application of bioinformatic data analysis as a core technology in the undergraduate Biology, Biochemistry and Bioinformatics curricula at USP. Microarray technology is an important biotechnology tool for the study of gene expression and in molecular diagnostics and has launched a paradigm shift in the way pharmaceutical and biotechnology industries approach drug discovery and development. An entire industry of microarray instrument, reagent and bioinformatic companies have developed to meet the current and future needs of the biological science community. Recently, instrumentation has been developed for making microarrays ("gridders") and microarray readers ("scanners") that are marketed and priced for the small laboratory environment. Students who graduate with a Life Science degree in the 21st century need to know the scientific principles and practical technological skills of microarray technology. Our cross-disciplinary curriculum program adapts microarray technology and develops microarray specific exercises in three courses, Molecular Biology (BI-290), Genetics (BS-466), Bioinformatics (BI-450) and supports undergraduate research in our Directed Research program (BS-499 and CH-450). This program is part of the educational foundation for our new Bachelor of Science degree in Bioinformatics and gives students in the Biology and Biochemistry programs the opportunity to experience microarray technology in their 2nd , 3rd or 4th years of study. Students carry out gene expression and DNA diagnostic experiments, and work with the bioinformatic software that is used to organize and mine microarray data. Successful implementation of this proposal is leading to the expansion of DNA microarray exercises to other courses and programs (eg. Microbiology). This project is providing a curricular template that leads to protocols and data sets for other undergraduate institutions.