Inheritance of Mitochondrial DNA in Didymiun iridis, an Isogamous, Coenocytic Organism with Multiple Sexes

Didymiun iridis 中线粒体 DNA 的遗传，一种同配异体、多性别的同核生物

• 批准号: 9305498
• 负责人: Margaret Silliker
• 金额: $ 16万
• 依托单位: DePaul University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-15 至 1996-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9305498&HistoricalAwards=false
• 关键词: Inheritance; Mitochondrial; DNA; Didymiun; iridis

9305498 Silliker These experiments will determine the pattern of inheritance of mitochondrial genomes in the acellular slime mold Didymiym iridis (Ditmar) Fries (class Myxomycetes). The mitochondrial DNA of one strain of D. iridis will be characterized by cloning and restriction enzyme mapping. Mitochondrial DNA variation will be assessed within strains of the largest heterothallic interbreeding group by comparison of probe hybridization patterns between strains. Once probes diagnostic for a mitochondrial type are identified they will be used to follow the fate of mitochondria in crosses. The results will be interpreted with special attention to the probable relatedness of these strains based on mitochondrial restriction fragment length polymorphism, geographical origin, and nuclear isozyme and mating type data. Uniparental organelle inheritance is an emerging rule for organelle behavior. Transmittance patterns are a result of unequal parental contribution (anisogamy) or mating type control in isogamous organisms with binary mating systems. Heterothallic myxomycetes have multiple-allelic mating systems. The pattern of mitochondrial inheritance in the myxomycete Physarum polycephalum was found to be determined by a mating type hierarchy. Preliminary evidence suggests that this is not the case with D. iridis. When compatible strains (different mating types) were crossed, about half the individuals inherited one mitochondrial type and half the other type. Replicate crosses with ten strains will be made in all possible combinations and the pattern of mitochondrial inheritance will be determined by hybridization of strain diagnostic probes to DNA samples from individuals. %%% Determining the pattern of mitochondrial transmittance in Didymium should allow development of hypotheses about the mechanism of inheritance. One working hypothesis predicts that mitochondrial inheritance will be more tightly regulated in closely related strains. The results of this research could be of major importance to evolutionary genetics. The involvement of undergraduate students in the project will be beneficial to the training of molecular geneticists. ***

9305498 Silliker这些实验将确定无细胞粘菌Didymiym iridis(Ditmar)Fries(粘菌纲)中线粒体基因组的遗传模式。对一株虹膜蠕形虫的线粒体DNA进行克隆和限制性内切酶图谱分析。线粒体DNA变异将通过比较不同品系之间的探针杂交模式来评估最大的异种杂交群体的品系之间的差异。一旦确定了线粒体类型的诊断探针，它们将被用来追踪交叉中线粒体的命运。根据线粒体限制性片段长度多态、地理来源、核同工酶和交配型数据，这些结果将特别注意这些菌株之间的可能亲缘关系。细胞器单亲遗传是细胞器行为的一种新规则。在具有二元交配系统的同配子生物中，透过性模式是亲本贡献不平等(异配)或交配型控制的结果。异株粘菌具有多等位基因的交配系统。多头绒泡菌的线粒体遗传模式被发现由交配型等级决定。初步证据表明，虹膜吸虫的情况并非如此。当相容的菌株(不同的交配型)杂交时，大约一半的个体遗传了一种线粒体类型，一半遗传了另一种类型。与10个菌株的重复杂交将在所有可能的组合中进行，线粒体遗传模式将通过菌株诊断探针与来自个体的DNA样本的杂交来确定。在Didymium中确定线粒体透过性的模式应该允许关于遗传机制的假说的发展。一种工作假说预测，线粒体遗传在密切相关的菌株中将受到更严格的调控。这项研究的结果可能对进化遗传学具有重要意义。本科生的参与将有利于分子遗传学家的培养。***