Dynamic Studies of the Adhesion, Deformation and Fusion of Monolayers, Bilayers and Membranes

单层、双层和膜的粘附、变形和融合的动态研究

• 批准号: 9305868
• 负责人: Jacob Israelachvili
• 金额: $ 28.5万
• 依托单位: University of California-Santa Barbara
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-01 至 1996-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9305868&HistoricalAwards=false
• 关键词: Dynamic; Studies; Adhesion; Deformation; Fusion

9305868 Israelachvili This project continues the investigation of the roles of different interbilayer forces and bilayers fluidity on the molecular-scale deformations of adhering and fusing bilayers using novel electron and atomic force microscopy and surface force measurements. Past experiments identified a particularly important role for hydrophobic forces in adhesion and fusion. In this follow-up project, a variety of techniques are used to study the dynamics of surfactants, lipids and domains (lateral diffusion, overturning or 'flip-flop', and exchange) during nonequilibrium interactions such as adhesion, fusion and membrane structural transitions. The aim is to asess how these dynamic processes and their rates - in contrast to equilibrium properties - affect structural changes, adhesion and fusion. The investigators will attempt to achieve similarly high time and spatial resolution of ionic effects on membrane interactions by using 'cages' calcium compounds to liberate divalent ions at a specific time and place. Complementary surface force apparatus and cyro-electron microscopy measurements will be made how calcium induces membrance structural changes, adhesion and fusion with millisecond time resolution. Additional structural information will be provided at the sub-molecular scale by studying the effects of divalent ions on simple Langmuir- Blodgett membranes using the atomic force microscope.

小行星9305868 该项目继续使用新型电子和原子力显微镜和表面力测量来研究不同的双分子层间力和双分子层流动性对粘附和融合双分子层的分子尺度变形的作用。 过去的实验确定了疏水力在粘附和融合中的特别重要的作用。 在这个后续项目中，各种技术被用来研究表面活性剂，脂质和域（横向扩散，翻转或'触发器'，和交换）的动态过程中， 非平衡相互作用，如粘附、融合和膜结构转变。 目的是评估这些动态过程及其速率-与平衡性质相比-如何影响结构变化，粘附和融合。 研究人员将尝试通过使用“笼”钙化合物在特定时间和地点释放二价离子来实现离子对膜相互作用的类似高时间和空间分辨率。 互补的表面力装置和冷冻电子显微镜测量将如何钙诱导膜结构的变化，粘附和融合与毫秒级的时间分辨率。 通过使用原子力显微镜研究二价离子对简单朗缪尔-布洛杰特膜的影响，将在亚分子尺度上提供额外的结构信息。