Arabidopsis DNA Methylation Mutants

拟南芥 DNA 甲基化突变体

• 批准号: 9306266
• 负责人: Eric Richards
• 金额: $ 34.5万
• 依托单位: Washington University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 1997-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9306266&HistoricalAwards=false
• 关键词: Arabidopsis; DNA; Methylation; Mutants

9306266 Richards Post-replicative methylation is the most common DNA modification present in eukaryotic cells. Although DNA methylation has been the subject of intense study, many questions remain about the function of this form of DNA modification in eukaryotes. The proposal outlines a genetic approach to the study of DNA methylation using the flowering plant Arabidopsis thaliana as a model. We have isolated A. thaliana mutants that contain reduced levels of cytosine methylation. Plans for further characterization of these hypomethylation mutants and strategies for isolating new methylation mutants are proposed. %%% It is anticipated that the A. thaliana mutants will provide a new system for studying eukaryotic DNA methylation. ***

理查兹行星9306266 复制后甲基化是真核细胞中最常见的DNA修饰。 虽然DNA甲基化一直是深入研究的主题，但关于这种形式的DNA修饰在真核生物中的功能仍然存在许多问题。 该提案概述了以开花植物拟南芥（Arabidopsis thaliana）为模型研究DNA甲基化的遗传方法。 分离出A。含有降低水平的胞嘧啶甲基化的拟南芥突变体。 提出了进一步表征这些低甲基化突变体的计划和分离新的甲基化突变体的策略。 %预计A.拟南芥突变体为真核生物DNA甲基化研究提供了一个新的体系。 ***