Optimization of Heterologous Protein Secretion from YarrowiaLipolytica: An Integrated Biomolecular and Bioprocess Engineering Approach

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9308822 Ryu This project is on research to develop an expression and secretion system for the yeast Yarrowia Lipolytica. The work involves extensive molecular biological studies which includes development of a transformation-secretion system, genetic analysis of a promoter, and isolation of a high secretion mutant. The developed strain will be used to express model proteins of industrial relevance, and a two stage fed-batch reactor system will be used to optimize expression levels.