Studies on the Reaction Kinetics and Mechanisms of Enzymes Involved in the Biosynthesis of B-Lactam Antibiotics Using Purified Enzymes

利用纯化酶研究 B-内酰胺类抗生素生物合成中酶的反应动力学和机理

• 批准号: 8611540
• 负责人: Dewey Ryu
• 金额: $ 12.97万
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-01 至 1989-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8611540&HistoricalAwards=false
• 关键词: Studies; Reaction; Kinetics; Mechanisms; Enzymes

This research is a fundamental biochemical engineering study on the purification, biochemical characterization, and the reaction kinetics and mechanisms of enzymes involved in the biosyntheses of beta-lactam antibiotics. The overall biochemical characteristics and basic reaction kinetics and mechanisms of those enzymes involved in the biosynthesis of beta-lactam antibiotics and further apply the knowledge base to be acquired from this research to the improvement of fermentation process and/or development of new and direct in-vitro enzymatic processes for production of existing and novel beta-lactam antibiotics derivatives. During the initial phase of the research, efforts will focus on the purification and characterization of those key enzymes involved in the ring formation and ring expansion reactions of beta-lactam antibiotics.

本研究是对β -内酰胺类抗生素生物合成过程中酶的纯化、生化表征、反应动力学和机理的基础生化工程研究。了解参与β -内酰胺类抗生素生物合成的酶的总体生化特性、基本反应动力学和机制，并进一步将本研究获得的知识基础应用于发酵工艺的改进和/或开发新的和直接的体外酶促工艺，以生产现有的和新的β -内酰胺类抗生素衍生物。在研究的初始阶段，将致力于纯化和表征β -内酰胺类抗生素的环形成和环扩张反应的关键酶。