Construction of PCB-Metabolizing Bacteria in Soil

9311144 Focht这是一个奖项，以支持研究，以确定是否互补的分解代谢途径内的细菌菌株的生物降解的柠檬酸异生质化学品，如多氯联苯可以纳入一个单一的途径，通过加强基因之间的交换添加和本地土壤细菌。 目前用于组合来自两种或更多种分解代谢表型的基因的策略需要分离亲本菌株和体外遗传操作。 在本研究中，研究人员计划将所需的氯苯甲酸盐利用生物添加到含有能够单独生物降解联苯的土著生物的土壤中，以确定目标底物的降解是否得到增强，并确定在本项目进行期间选择和开发的不同杂交菌株的相对有效性。 这项研究的结果预计将提供深入了解如何已被污染的土壤和沉积物多氯联苯可以净化使用的生物体能够利用这些有机化合物作为代谢底物。 这些知识可用于生物反应过程的工程设计和土壤的原位净化。

9311144 Focht This is an award to support research to determine whether complementary catabolic pathways within bacterial strains for biodegradation of recalcitrant xenobiotic chemicals such as the polychlorinated biphenyls can be incorporated into a single pathway by enhancement of genetic exchange between added and indigenous soil bacteria. Current strategies for combining the genes from two or more catabolic phenotypes requires isolation of parental strains and genetic manipulation in vitro. In this research, the investigator plans on adding the requisite chlorobenzoate utilizer organisms to soil containing indigenous organisms capable of biodegrading biphenyl alone to determine whether degradation of the target substrate is enhanced and to determine the relative effectiveness of the different hybrid strains selected and developed during conduct of this project. Results of this research are expected to provide insights into how soil and sediments that have been contaminated with polychlorinated biphenyls can be decontaminated by use of organisms capable of utilizing these organic compounds as metabolic substrates. This knowledge may be utilized in engineering design of bioreaction processes and for in situ decontamination of soil.