SGER: Iron Fertilization of Oceanic Phytoplankton: A Molecular of Chloroplast Gene Response

SGER：海洋浮游植物的铁施肥：叶绿体基因反应的分子

• 批准号: 9314260
• 负责人: Rose Ann Cattolico
• 金额: $ 5万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-07-15 至 1995-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9314260&HistoricalAwards=false
• 关键词: SGER; Iron; Fertilization; Oceanic; Phytoplankton

Iron plays a critical role in the maintenance of cell metabolism as phytoplankton appear to regulate intracellular iron concentrations. However, at present no one has conducted molecular studies on the effects of iron deprivation on the genes that encode proteins of photosystem II, Calvin cycle enzyme, and light harvesting chlorophyll. This project will determine how chloroplast gene expression is regulated by iron (Fe++) in the eukaryotic marine alga, Olisthodiscus luteus. This alga, which is ecologically significant in toxic algal blooms yet essentially unstudied at the molecular level, will serve as a model system to address critical physiological and ecological questions in other marine algae. The work will provide critical information on how specific genes regulate these proteins at the transcription or post-transcription level in response to iron as an environmental cue. If successful, future work will focus on factors that impose transcription control or those that influence RNA stability and/or selective utilization of specific RNA species.

铁在维持细胞代谢中起着至关重要的作用，浮游植物似乎可以调节细胞内铁的浓度。然而，目前还没有人对缺铁对光系统II蛋白编码基因、卡尔文循环酶和捕光叶绿素蛋白的影响进行分子研究。本项目将确定真核藻类Olisthodiscus luteus中铁（Fe++）如何调节叶绿体基因表达。这种藻类在有毒藻华中具有重要的生态学意义，但在分子水平上基本上尚未进行研究，它将作为解决其他海洋藻类关键生理和生态问题的模型系统。这项工作将提供关于特定基因如何在转录或转录后水平上调节这些蛋白质以响应铁作为环境线索的关键信息。如果成功，未来的工作将集中在施加转录控制或影响RNA稳定性和/或特定RNA物种选择性利用的因素上。