Chloroplast Signal Transduction in Marine Chromophytes

海洋有色植物中的叶绿体信号转导

• 批准号: 9728262
• 负责人: Rose Ann Cattolico
• 金额: $ 8.8万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-02-15 至 2000-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9728262&HistoricalAwards=false
• 关键词: Chloroplast; Signal; Transduction; Marine; Chromophytes

MCB9728262 Cattolico, Rose Ann Chloroplast Signal Transduction in Marine Chromophytes Lay Summary Management of coastal ecosystems has recently become a major topic for the national news media. There is a new awareness of the contribution by phytoplankton to a balanced marine ecology. Part of this awareness has been gained from observing the devastating effects of algal blooms off the coast of the United States. Phytoplankton, however, also serve as the keystone of aquatic food webs and produce fascinating compounds of biomedical importance which can be "mined" from the sea. Algae live in a precarious environment and must respond rapidly to changing environmental cues (e.g., light, temperature, and nutrients) to ensure survival. Our study analyzes a mechanism by which a potentially toxic photosynthetic alga perceives these cues and transduces those signals into metabolic commands. Specifically, we will study the effector component of a prokaryotic-like signal transduction system that is localized in the chloroplast. This component modulates gene expression by affecting transcription. We have sequenced the gene that produces the effector protein. Next we will overproduce this protein in a bacterial expression system and then analyze the genes that are targeted by the protein. Finally, we hope to identify the receptor of this signal transduction cascade. This research when completed will provide reference information for a taxon (Chromophyta) that is ecologically important yet has received only marginal scientific attention.

MCB9728262 Cattolico，Rose Ann 海洋色藻中的叶绿体信号转导概要 沿海生态系统的管理最近已成为国家新闻媒体的主要话题。 人们对浮游植物对平衡海洋生态的贡献有了新的认识。 这种认识的一部分是通过观察美国海岸藻华的破坏性影响而获得的。 然而，浮游植物也是水生食物网的基石，并产生具有生物医学重要性的令人着迷的化合物，这些化合物可以从海洋中“开采”。 藻类生活在不稳定的环境中，必须对不断变化的环境线索（例如光、温度和营养物质）做出快速反应，以确保生存。 我们的研究分析了一种机制，潜在有毒的光合藻类感知这些线索并将这些信号转换为代谢命令。 具体来说，我们将研究位于叶绿体中的原核样信号转导系统的效应器成分。 该成分通过影响转录来调节基因表达。 我们已经对产生效应蛋白的基因进行了测序。 接下来，我们将在细菌表达系统中过量产生这种蛋白质，然后分析该蛋白质所针对的基因。 最后，我们希望能够鉴定出该信号转导级联的受体。 这项研究完成后将为一个分类单元（有色植物）提供参考信息，该分类单元在生态上很重要，但只受到了很少的科学关注。