Development of High Resolution X-Ray Microscopy

一台扫描软x射线显微镜在布鲁克海文国家同步加速器光源实验室建成并投入运行。该仪器在X1A光束线上使用明亮、可调谐的波动源。它使用劳伦斯伯克利实验室制造的带板。与IBM合作，形成50纳米的光束光斑。显微镜在常规操作中，由几个小组使用，以光斑大小设定的分辨率对湿生物标本进行成像。正在开发新的成像模式，以扩展该技术的适用性，利用元素吸收边缘（XANES）附近存在的共振来绘制特定的化学状态，并利用x射线束检测荧光标签中激发的可见光。除了继续显微镜的运行外，还要求支持扩展XANES对比成像能力，大幅提高仪器的稳定性和分辨率，并探索更高能量下x射线显微镜的可能性。该小组欢迎有兴趣使用该仪器解决与其独特功能相匹配的生物学问题的研究人员的询问。U ~ s &t &9:；= ?@ A B C D E F G H I J K L M N O P R S T U V W X X Y Z \ K _ A A B B C D X X X X X X X X射线扫描显微镜在国家同步光源建成并投入运行。b pvx ^ (b) ！！ ！ ！ F b p b 3 Times New Roman Symbol & Arial 1 Courier L " h ee = abstract Deseree King, BIR Deseree King， BIR

9316594 Kirz A scanning soft x ray microscope has been built and put into operation at the National Synchrotron Light Source, Brookhaven National Laboratory. The instrument makes use of the bright, tuneable undulator source at the X1A beamline. It uses zone plates fabricated under a Lawrence Berkeley Lab./IBM collaboration to form a 50 nm beam spot. The microscope is in routine operation, and is used by several groups to image wet biological specimens at the resolution set by the spot size. New imaging modes are being developed that extend the applicability of the technique to map particular chemical states using the resonances present near elemental absorption edges (XANES), and using the detection of visible light excited in fluorescent labels by the x ray beam. In addition to continuing the operation of the microscope, support is requested to extend the XANES contrast imaging capability, to improve substantially the stability and the resolution of the instrument, and to explore the possibilities in x ray microscopy at higher energies. The team welcomes inquiries by investigators interested in using the instrument to solve biological problems matched to its unique capabilities. u ~ s &t &9 : ; = ? @ A B C D E F G H I J K L M N O P R S T U V W X Y Z \ K _ ` a b c d e f g h i j k r m o I t u v w x y { | ~ 9316594 Kirz A scanning soft x ray microscope has been built and put into operation at the National Synchrot ron Light Source, b p v x ^ ` b ! ! ! ! F b p b 3 Times New Roman Symbol & Arial 1 Courier L " h e e = abstract Deseree King, BIR Deseree King, BIR